Recombinant mouse CD40 protein (Active) is a Mouse Fragment protein, in the 24 to 193 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
V T C S D K Q Y L H D G Q C C D L C Q P G S R L T S H C T A L E K T Q C H P C D S G E F S A Q W N R E I R C H Q H R H C E P N Q G L R V K K E G T A E S D T V C T C K E G Q H C T S K D C E A C A Q H T P C I P G F G V M E M A T E T T D T V C H P C P V G F F S N Q S S L F E K C Y P W T S C E D K N L E V L Q K G T S Q T N V I C G L K S R M R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:12881420).
Alternative names
CD40, Tnfrsf5, Cd40, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor
Recommended products
Recombinant mouse CD40 protein (Active) is a Mouse Fragment protein, in the 24 to 193 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized ab220566 at 5μg/mL (100 μL/well) can bind Mouse CD40 Ligand, Fc Tag with a linear range of 1-16 ng/mL.
- Accession
- P27512-1
- Animal free
- No
- Species
- Mouse
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- V T C S D K Q Y L H D G Q C C D L C Q P G S R L T S H C T A L E K T Q C H P C D S G E F S A Q W N R E I R C H Q H R H C E P N Q G L R V K K E G T A E S D T V C T C K E G Q H C T S K D C E A C A Q H T P C I P G F G V M E M A T E T T D T V C H P C P V G F F S N Q S S L F E K C Y P W T S C E D K N L E V L Q K G T S Q T N V I C G L K S R M R
- Accession
- P27512
- Protein length
- Fragment
- Predicted molecular weight
- 20.8 kDa
- Amino acids
- 24 to 193
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:12881420).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Recombinant mouse CD40 protein (Active) (ab220566)
Immobilized ab220566 at 5μg/mL (100 μL/well) can bind Mouse CD40 Ligand, Fc Tag with a linear range of 1-16 ng/mL.
SDS-PAGE - Recombinant mouse CD40 protein (Active) (ab220566)
SDS-PAGE analysis of reduced ab220566 stained overnight with Coomassie Blue.
The protein migrates as 30 kDa on a SDS-PAGE gel under reducing conditions due to glycosylation.
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Product protocols
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