Recombinant Mouse CD53 protein (His tag) is a Mouse Fragment protein, in the 107 to 181 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
E Q K L N T L V A E G L N D S I Q H Y H S D N S T M K A W D F I Q T Q L Q C C G V N G S S D W T S G P P S S C P S G A D V Q G C Y N K A K S W F H S N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed:36581203). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed:32428859, PubMed:32532837). Plays a role in the activation of monocytes and B-cells (By similarity). Acts as an essential regulator of B-cell development by promoting interleukin-7 receptor/IL7R signaling (PubMed:31748347). Promotes also in B-cells the BCR signaling by recruiting PKC to the plasma membrane in order to phosphorylate its substrates (By similarity). Plays an essential role in lymphocyte homing to lymph nodes by stabilizing L-selectin/SELL cell surface expression (PubMed:32428859). Mediates also metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF-alpha and LPS signaling independent of the immune compartment (PubMed:36581203). Protects hematopoietic stem cell function in response to stress by facilitating DREAM complex activity through association with p130/RBL2 and its phosphatase PP2A (PubMed:36542833).
Alternative names
CD53, Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Cd53
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Recombinant Mouse CD53 protein (His tag) is a Mouse Fragment protein, in the 107 to 181 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q61451-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- E Q K L N T L V A E G L N D S I Q H Y H S D N S T M K A W D F I Q T Q L Q C C G V N G S S D W T S G P P S S C P S G A D V Q G C Y N K A K S W F H S N
- Accession
- Q61451
- Protein length
- Fragment
- Predicted molecular weight
- 10.6 kDa
- Amino acids
- 107 to 181
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed:36581203). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed:32428859, PubMed:32532837). Plays a role in the activation of monocytes and B-cells (By similarity). Acts as an essential regulator of B-cell development by promoting interleukin-7 receptor/IL7R signaling (PubMed:31748347). Promotes also in B-cells the BCR signaling by recruiting PKC to the plasma membrane in order to phosphorylate its substrates (By similarity). Plays an essential role in lymphocyte homing to lymph nodes by stabilizing L-selectin/SELL cell surface expression (PubMed:32428859). Mediates also metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF-alpha and LPS signaling independent of the immune compartment (PubMed:36581203). Protects hematopoietic stem cell function in response to stress by facilitating DREAM complex activity through association with p130/RBL2 and its phosphatase PP2A (PubMed:36542833).
- Sequence similarities
Belongs to the tetraspanin (TM4SF) family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD53 also known as Tetraspanin-25 is a transmembrane glycoprotein with a molecular mass of approximately 35-40 kDa. It belongs to the tetraspanin family characterized by four transmembrane domains. CD53 primarily resides on the surface of leukocytes including B cells T cells natural killer cells and macrophages. This protein plays an important role in signal transduction processes by organizing proteins on the cell membrane and forming tetraspanin-enriched microdomains.
- Biological function summary
CD53 plays a significant role in regulating immune responses cell development and migration. It does not work alone but as part of larger protein complexes that influence cell communication and inter-organizational responses. CD53 participates in organizing membrane proteins into functional complexes which impacts cellular processes such as adhesion signaling and cellular activation. Its interaction with other proteins is critical to maintain normal immune system functionality and responding appropriately to infections.
- Pathways
CD53 is involved in the regulation of immune signaling and cellular communication networks. It is a component of the tetraspanin web which influences the integrin and immunoreceptor pathways two important signaling pathways in cellular communication. Proteins such as CD9 and CD81 also members of the tetraspanin family frequently interact with CD53 suggesting cooperative roles in modulating cellular adhesion and trafficking processes critical for immune response.
- Associated diseases and disorders
CD53 shows a connection to immune system dysfunctions including autoimmune conditions and certain types of cancer. Its altered expression can contribute to abnormal immune responses seen in diseases like systemic lupus erythematosus (SLE) and lymphoma. In these disorders CD53's interaction with CD19 and other B-cell-associated proteins plays a part in abnormal cell signaling leading to issues in cell proliferation or miscommunication within the immune system.
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1 product image
SDS-PAGE - Recombinant Mouse CD53 protein (His tag) (ab276914)
SDS-PAGE analysis of ab276914
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