Recombinant Mouse CD73 protein (His tag) is a Mouse Fragment protein, in the 29 to 551 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE, HPLC.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- MS, SDS-PAGE, HPLC
- Biologically active
- No
Amino acid sequence
W E L T I L H T N D V H S R L E Q T S D D S T K C L N A S L C V G G V A R L F T K V Q Q I R K E E P N V L F L D A G D Q Y Q G T I W F T V Y K G L E V A H F M N I L G Y D A M A L G N H E F D N G V E G L I D P L L R N V K F P I L S A N I K A R G P L A H Q I S G L F L P S K V L S V G G E V V G I V G Y T S K E T P F L S N P G T N L V F E D E I S A L Q P E V D K L K T L N V N K I I A L G H S G F E M D K L I A Q K V R G V D I V V G G H S N T F L Y T G N P P S K E V P A G K Y P F I V T A D D G R Q V P V V Q A Y A F G K Y L G Y L K V E F D D K G N V I T S Y G N P I L L N S S I P E D A T I K A D I N Q W R I K L D N Y S T Q E L G R T I V Y L D G S T Q T C R F R E C N M G N L I C D A M I N N N L R H P D E M F W N H V S M C I V N G G G I R S P I D E K N N G T I T W E N L A A V L P F G G T F D L V Q L K G S T L K K A F E H S V H R Y G Q S T G E F L Q V G G I H V V Y D I N R K P W N R V V Q L E V L C T K C R V P I Y E P L E M D K V Y K V T L P S Y L A N G G D G F Q M I K D E L L K H D S G D Q D I S V V S E Y I S K M K V V Y P A V E G R I K F S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside (PubMed:8224905). Hydrolyzes IMP (PubMed:8224905). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (By similarity). Although AMP is the preferred substrate can also hydrolyze UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (By similarity).
Alternative names
CD73, Nt5, Nte, Nt5e, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase
Recommended products
Recombinant Mouse CD73 protein (His tag) is a Mouse Fragment protein, in the 29 to 551 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE, HPLC.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- MS, SDS-PAGE, HPLC
- Biologically active
- No
- Accession
- Q61503-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- W E L T I L H T N D V H S R L E Q T S D D S T K C L N A S L C V G G V A R L F T K V Q Q I R K E E P N V L F L D A G D Q Y Q G T I W F T V Y K G L E V A H F M N I L G Y D A M A L G N H E F D N G V E G L I D P L L R N V K F P I L S A N I K A R G P L A H Q I S G L F L P S K V L S V G G E V V G I V G Y T S K E T P F L S N P G T N L V F E D E I S A L Q P E V D K L K T L N V N K I I A L G H S G F E M D K L I A Q K V R G V D I V V G G H S N T F L Y T G N P P S K E V P A G K Y P F I V T A D D G R Q V P V V Q A Y A F G K Y L G Y L K V E F D D K G N V I T S Y G N P I L L N S S I P E D A T I K A D I N Q W R I K L D N Y S T Q E L G R T I V Y L D G S T Q T C R F R E C N M G N L I C D A M I N N N L R H P D E M F W N H V S M C I V N G G G I R S P I D E K N N G T I T W E N L A A V L P F G G T F D L V Q L K G S T L K K A F E H S V H R Y G Q S T G E F L Q V G G I H V V Y D I N R K P W N R V V Q L E V L C T K C R V P I Y E P L E M D K V Y K V T L P S Y L A N G G D G F Q M I K D E L L K H D S G D Q D I S V V S E Y I S K M K V V Y P A V E G R I K F S
- Accession
- Q61503
- Protein length
- Fragment
- Predicted molecular weight
- 59.6 kDa
- Amino acids
- 29 to 551
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE>=95%
General info
- Function
Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside (PubMed:8224905). Hydrolyzes IMP (PubMed:8224905). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (By similarity). Although AMP is the preferred substrate can also hydrolyze UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (By similarity).
- Sequence similarities
Belongs to the 5'-nucleotidase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD73 also known as ecto-5'-nucleotidase is an enzyme that functions at the cellular level. The CD73 protein hydrolyzes extracellular nucleotides into nucleosides by removing phosphate groups. It is a 70 kDa glycosylphosphatidylinositol-anchored molecule found on the surface of various cell types including endothelial cells lymphocytes and epithelial cells. Its presence is noted in a wide range of tissues across the human body highlighting its versatile role in cellular communication and metabolism.
- Biological function summary
CD73 participates in the regulation of purinergic signaling through adenosine production. It acts in cooperation with other cell surface enzymes such as CD39 forming a functional complex that handles ATP breakdown to adenosine. This enzymatic activity modulates immune responses tissue protection and inflammation control. In addition to immune-related functions CD73 supports the maintenance of vascular integrity and proper cellular adhesion which is important in different physiological and pathological contexts.
- Pathways
CD73 plays an important part in the adenosinergic pathway governing the conversion of AMP into adenosine. This activity impacts the signaling and function of the adenosine receptors on immune and non-immune cells. Another important pathway is the angiogenesis process where CD73 contributes by affecting endothelial cell migration and blood vessel development. Relationships with key proteins such as adenosine A2 receptors help facilitate these critical pathway activities.
- Associated diseases and disorders
CD73's involvement with immunosuppression and tumor progression becomes significant. Elevated levels of CD73 are frequently observed in some cancers where it aids in creating a favorable tumor microenvironment through increased adenosine production. This situation assists tumor cells in evading immune detection. Additionally CD73 is linked to autoimmune diseases like multiple sclerosis where it contributes to modulated lymphocyte activity. Connections with proteins such as TGF-beta further illustrate its role in disease-related pathways creating potential targets for therapeutic interventions through the use of anti-CD73 antibodies.
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2 product images
HPLC - Recombinant Mouse CD73 protein (His tag) (ab310790)
HPLC analysis of ab310790
SDS-PAGE - Recombinant Mouse CD73 protein (His tag) (ab310790)
SDS-PAGE analysis of ab310790
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