Recombinant Mouse CD8 alpha + CD8B protein (Tagged) is a Mouse Fragment protein, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
M Q P W L W L V F S M K L A A L W S S S A L I Q T P S S L L V Q T N H T A K M S C E V K S I S K L T S I Y W L R E R Q D P K D K Y F E F L A S W S S S K G V L Y G E S V D K K R N I I L E S S D S R R P F L S I M N V K P E D S D F Y F C A T V G S P K M V F G T G T K L T V V D V L P T T A P T K K T T L K M K K K K Q C P F P H P E T Q K G L T C S L T T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Additional Targets
Cd8b
Alternative names
CD8a, Lyt-2, Lyt2, Cd8a, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2
Recommended products
Recombinant Mouse CD8 alpha + CD8B protein (Tagged) is a Mouse Fragment protein, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P01731-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence 1
- Accession
- P01731
- Protein length
- Fragment
- Predicted molecular weight
- 23.7 kDa
- Nature
- Recombinant
Amino acid sequence 2
- M Q P W L W L V F S M K L A A L W S S S A L I Q T P S S L L V Q T N H T A K M S C E V K S I S K L T S I Y W L R E R Q D P K D K Y F E F L A S W S S S K G V L Y G E S V D K K R N I I L E S S D S R R P F L S I M N V K P E D S D F Y F C A T V G S P K M V F G T G T K L T V V D V L P T T A P T K K T T L K M K K K K Q C P F P H P E T Q K G L T C S L T T
- Accession
- P10300
- Protein length
- Fragment
- Predicted molecular weight
- 22.2 kDa
- Amino acids
- 1 to 175
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
- Post-translational modifications
Palmitoylated, but association with CD8B seems to be more important for the enrichment of CdD8A in lipid rafts.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD8 alpha and CD8 beta are proteins that form part of the CD8 co-receptor complex. These proteins work together to perform critical functions in the immune system by facilitating interactions with the major histocompatibility complex (MHC) class I molecules on the surface of antigen-presenting cells. The CD8 alpha (CD8A) and CD8 beta (CD8B) proteins have molecular weights of approximately 32 kDa and 37 kDa respectively. They are expressed mainly on cytotoxic T cells some natural killer cells and subsets of dendritic cells playing an important role in immune response.
- Biological function summary
The CD8A and CD8B proteins function as a dimer to assist T-cell receptor (TCR) triggering and enhance TCR signaling. This interaction helps the immune system recognize and eliminate virus-infected cells and tumor cells. By forming a part of the CD8 co-receptor complex these proteins contribute to the specificity of the T-cell mediated immune response by stabilizing MHC class I-TCR interactions which ensures effective immune surveillance.
- Pathways
CD8 alpha and CD8 beta proteins participate in the antigen processing and presentation pathway. This pathway involves their interaction with MHC class I molecules that are important for T-cell activation and differentiation. The CD8 co-receptor complex works closely with the TCR complex to ensure the appropriate immune responses. The involvement of proteins such as Lck a tyrosine kinase is essential in this pathway highlighting the collaborative network of proteins working together for immune function.
- Associated diseases and disorders
CD8 alpha and CD8 beta associations highlight their role in autoimmune diseases like multiple sclerosis and in various cancers. Aberrations in CD8 expression or function can result in either inadequate immune responses or inappropriate targeting of self-antigens. The relationship with proteins like PD-1 which is involved in immune checkpoint regulation indicates the relevance of CD8 proteins in developing therapeutic strategies for these conditions.
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1 product image
SDS-PAGE - Recombinant Mouse CD8 alpha + CD8B protein (Tagged) (ab277035)
SDS-PAGE analysis of ab277035
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