Recombinant Mouse CD80 protein (His tag) is a Mouse Fragment protein, in the 37 to 246 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for FuncS, SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- FuncS, SDS-PAGE
- Biologically active
- No
Amino acid sequence
D V D E Q L S K S V K D K V L L P C R Y N S P H E D E S E D R I Y W Q K H D K V V L S V I A G K L K V W P E Y K N R T L Y D N T T Y S L I I L G L V L S D R G T Y S C V V Q K K E R G T Y E V K H L A L V K L S I K A D F S T P N I T E S G N P S A D T K R I T C F A S G G F P K P R F S W L E N G R E L P G I N T T I S Q D P E S E L Y T I S S Q L D F N T T R N H T I K C L I K Y G D A H V S E D F T W E K P P E D P P D S K N H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed:22450810).
Alternative names
CD80, B7, Cd80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen
Recommended products
Recombinant Mouse CD80 protein (His tag) is a Mouse Fragment protein, in the 37 to 246 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for FuncS, SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- FuncS, SDS-PAGE
- Biologically active
- No
- Accession
- Q00609-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- D V D E Q L S K S V K D K V L L P C R Y N S P H E D E S E D R I Y W Q K H D K V V L S V I A G K L K V W P E Y K N R T L Y D N T T Y S L I I L G L V L S D R G T Y S C V V Q K K E R G T Y E V K H L A L V K L S I K A D F S T P N I T E S G N P S A D T K R I T C F A S G G F P K P R F S W L E N G R E L P G I N T T I S Q D P E S E L Y T I S S Q L D F N T T R N H T I K C L I K Y G D A H V S E D F T W E K P P E D P P D S K N H H H H H H
- Accession
- Q00609
- Protein length
- Fragment
- Predicted molecular weight
- 24.7 kDa
- Amino acids
- 37 to 246
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed:22450810).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
- Biological function summary
CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.
- Pathways
CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.
- Associated diseases and disorders
CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
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2 product images
SDS-PAGE - Recombinant Mouse CD80 protein (His tag) (ab222422)
15% SDS-PAGE analysis of 3 μg ab222422.
MWt 28-40 kDa (SDS-PAGE under non-reducing conditions).
Functional Studies - Recombinant Mouse CD80 protein (His tag) (ab222422)
ab222422 is coated at 2 µg/ml (100 µl/well) can bind Mouse CTLA4 in a Functional ELISA assay.
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