Recombinant Mouse CD80 protein (Tagged) (Biotin) is a Mouse Fragment protein, in the 20 to 211 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
Amino acid sequence
F Q L L V L A G L S H F C S G V I H V T K E V K E V A T L S C G H N V S V E E L A Q T R I Y W Q K E K K M V L T M M S G D M N I W P E Y K N R T I F D I T N N L S I V I L A L R P S D E G T Y E C V V L K Y E K D A F K R E H L A E V T L S V K A D F P T P S I S D F E I P T S N I R R I I C S T S G G F P E P H L S W L E N G E E L N A I N T T V S Q D P E T E L Y A V S S K L D F N M T T N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed:22450810).
Alternative names
CD80, B7, Cd80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen
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Recombinant Mouse CD80 protein (Tagged) (Biotin) is a Mouse Fragment protein, in the 20 to 211 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
- Accession
- Q00609-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- F Q L L V L A G L S H F C S G V I H V T K E V K E V A T L S C G H N V S V E E L A Q T R I Y W Q K E K K M V L T M M S G D M N I W P E Y K N R T I F D I T N N L S I V I L A L R P S D E G T Y E C V V L K Y E K D A F K R E H L A E V T L S V K A D F P T P S I S D F E I P T S N I R R I I C S T S G G F P E P H L S W L E N G E E L N A I N T T V S Q D P E T E L Y A V S S K L D F N M T T N
- Accession
- Q00609
- Protein length
- Fragment
- Predicted molecular weight
- 52 kDa
- Amino acids
- 20 to 211
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed:22450810).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
- Biological function summary
CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.
- Pathways
CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.
- Associated diseases and disorders
CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
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1 product image
SDS-PAGE - Recombinant Mouse CD80 protein (Tagged) (Biotin) (ab271644)
SDS-PAGE analysis of ab271644.
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