Recombinant Mouse CD86 protein (His tag) is a Mouse Fragment protein, in the 24 to 244 aa range, expressed in Mammalian, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Mammalian
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
V S V E T Q A Y F N G T A Y L P C P F T K A Q N I S L S E L V V F W Q D Q Q K L V L Y E H Y L G T E K L D S V N A K Y L G R T S F D R N N W T L R L H N V Q I K D M G S Y D C F I Q K K P P T G S I I L Q Q T L T E L S V I A N F S E P E I K L A Q N V T G N S G I N L T C T S K Q G H P K P K K M Y F L I T N S T N E Y G D N M Q I S Q D N V T E L F S I S N S L S L S F P D G V W H M T V V C V L E T E S M K I S S K P L N F T Q E F P S P Q T Y W K H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (PubMed:23241883).
Alternative names
T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, Cd86
Recommended products
Recombinant Mouse CD86 protein (His tag) is a Mouse Fragment protein, in the 24 to 244 aa range, expressed in Mammalian, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Alternative names
T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, Cd86
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Mammalian
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P42082-1
- Animal free
No
- Carrier free
Yes
- Species
Mouse
- Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Reconstitute with ddH2O to a concentration no less than 100 µg/mL. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
V S V E T Q A Y F N G T A Y L P C P F T K A Q N I S L S E L V V F W Q D Q Q K L V L Y E H Y L G T E K L D S V N A K Y L G R T S F D R N N W T L R L H N V Q I K D M G S Y D C F I Q K K P P T G S I I L Q Q T L T E L S V I A N F S E P E I K L A Q N V T G N S G I N L T C T S K Q G H P K P K K M Y F L I T N S T N E Y G D N M Q I S Q D N V T E L F S I S N S L S L S F P D G V W H M T V V C V L E T E S M K I S S K P L N F T Q E F P S P Q T Y W K H H H H H H
- Accession
- Protein length
Fragment
- Predicted molecular weight
25.9 kDa
- Amino acids
24 to 244
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (PubMed:23241883).
- Post-translational modifications
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
- Biological function summary
CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.
- Pathways
CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.
- Associated diseases and disorders
CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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SDS-PAGE - Recombinant Mouse CD86 protein (His tag) (ab215653)
SDS Page analysis of ab215653: Lane 1: reducing conditions; Lane 2: non reducing conditions.
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