Recombinant Mouse CHIT1 Protein Standard (His tag) is a Mouse Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.
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Key facts
- Purity
>=80% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, sELISA
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens (By similarity).
Alternative names
Chitotriosidase-1, Chitinase-1, Chit1
Recommended products
Recombinant Mouse CHIT1 Protein Standard (His tag) is a Mouse Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.
Alternative names
Chitotriosidase-1, Chitinase-1, Chit1
Key facts
- Purity
>=80% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, sELISA
- Biologically active
No
- Accession
- Q9D7Q1-1
- Animal free
Yes
- Species
Mouse
- Concentration
- Storage buffer
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Accession
- Protein length
Fragment
- Predicted molecular weight
50.2 kDa
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Liquid
General info
- Function
Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens (By similarity).
- Sequence similarities
Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
- Subcellular localisation
Lysosome
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CHIT1 or chitotriosidase is a glycosyl hydrolase enzyme with significant roles in chitin breakdown. It has an approximate mass of 50 kDa and recognizes chitin-containing substances. CHIT1 predominantly expresses in macrophages and acts as an important enzyme in innate immunity by degrading pathologically accumulated chitin. Alternative names for CHIT1 include chitinase-1 and it is distinguishable by its active role in neutralizing fungal pathogens utilizing this chitinase mechanism.
- Biological function summary
CHIT1 participates in inflammatory processes and helps in tissue remodeling which is important in immune responses. CHIT1 does not typically form part of a complex but operates independently to mediate macrophage response through its chitinase activity. It handles polysaccharide elements in cellular debris impacting macrophage activation states. Macrophages utilize CHIT1 to counteract foreign bodies engulfed during phagocytosis playing essential roles in managing fungal infections.
- Pathways
CHIT1 influences both the immune response and metabolic pathways through its enzyme function. One key pathway involving CHIT1 is the mannose receptor-mediated clearance pathway which clears glycoproteins from systemic circulation. CHIT1 activity mirrors that of other chitinases like AMCase although they derive from separate genetic backgrounds. CHIT1 contribution enhances macrophage functionality within phagolysosomal pathways important for broad-spectrum pathogen eradication.
- Associated diseases and disorders
CHIT1 correlates significantly with Gaucher's disease and chronic obstructive pulmonary disease (COPD). In Gaucher's disease elevated CHIT1 levels serve as a biomarker indicating macrophage activation and infiltration. In the case of COPD CHIT1 modulates inflammatory responses that contribute to disease progression and respiratory complications. CHIT1 interacts with proteins associated with immune regulation such as TNF-alpha in these diseases impacting inflammatory pathways and disease severities.
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2 product images
Sandwich ELISA - Recombinant Mouse CHIT1 Protein Standard (His tag) (ab322282)
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
SDS-PAGE - Recombinant Mouse CHIT1 Protein Standard (His tag) (ab322282)
SDS-PAGE analysis of ab322282 under reducing conditions for 2ug protein.
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