Recombinant Mouse CHL1 protein (His tag) is a Mouse Fragment protein, in the 1 to 1027 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M M E L P L C G R G L I L S L I F L L L K L S A A E I P L S V Q Q V P T I V K Q S Y V Q V A F P F D E Y F Q I E C E A K G N P E P I F S W T K D D K P F D L S D P R I I A A N N S G T F K I P N E G H I S H F Q G K Y R C F A S N R L G T A V S E E I E F I V P G V P K F P K E K I E P I D V E E G D S I V L P C N P P K G L P P L H I Y W M N I E L E H I E Q D E R V Y M S Q R G D L Y F A N V E E N D S R N D Y C C F A A F P K L R T I V Q K M P M K L T V N S S N S I K Q R K P K L L L P P A Q M G S L S A K T V L K G D T L L L E C F A E G L P T P H I Q W S K P G S E L P E G R A T I E V H E K T L K I E N I S Y Q D R G N Y R C T A N N L L G K A S H D F H V T V E E P P R W K K K P Q S A V Y S T G S S G I L L C E A E G E P Q P T I K W R L N G L P I E K H P F P G D F M F P R E I S F T N L L P N H T G V Y Q C E A S N I H G T I L A N A N I D V I D V I P L I K T K N E E N Y A T V V G Y S A F L H C E Y F A S P K A T V V W E V A D E T H P L E G D R Y H T H E N G T L E I Y R T T E E D A G S Y S C W V D N A M G K A V I T A N L D I R N A T K L R V S P K N P R I P K S H V L E L Y C E S Q C D S H L K H S L K L S W S K D G E A F E M N G T E D G R I V I D G A Y L T I S N I T A E D Q G V Y S C S A Q T S L D S T S E K T Q V T V L G V P D P P G N L H L S E R Q N R S V R L S W E A G D D H N S K I S E Y I V E F E G N R E E P G K W E E L T R V Q G E E T D V V L S L A P Y V R Y Q F R V T A V N E V G R S H A S L P S D H H E T P P A A P D K N P Q N I R V Q A S Q P K E M I I K W E P L K S M E Q N G P G L E Y K V S W K P Q G A P E E W E E E I V T N H T L R V M T P T V Y A P Y D V K V Q A I N Q L G S S P D P Q P V T L Y S G E D Y P S T A P V I Q R V D V M N S T L V K V T W S S I P K E T V H G L L R G Y Q I N W W K T K S L L D G R T H P K E V N I L R F S G Q R N S G M V P S L D P F S E F H L T V L A Y N S K G A G P E S E P Y I F Q T P E G V P E Q P S F L K V I K V D K D T A T L S W G L P K K L N G N L T G Y L L Q Y Q I I N D T Y E L G E L N E I N V T T P S K S S W H L S N L N S T T K Y K F Y L R A C T S R G C G K P I S E E G A T L G E G S K G I R K I T E G V N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons as well as in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane.
Alternative names
Call, Chl1, Neural cell adhesion molecule L1-like protein, Cell adhesion molecule with homology to L1CAM, Chl1-like protein, Close homolog of L1
Recommended products
Recombinant Mouse CHL1 protein (His tag) is a Mouse Fragment protein, in the 1 to 1027 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P70232-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M M E L P L C G R G L I L S L I F L L L K L S A A E I P L S V Q Q V P T I V K Q S Y V Q V A F P F D E Y F Q I E C E A K G N P E P I F S W T K D D K P F D L S D P R I I A A N N S G T F K I P N E G H I S H F Q G K Y R C F A S N R L G T A V S E E I E F I V P G V P K F P K E K I E P I D V E E G D S I V L P C N P P K G L P P L H I Y W M N I E L E H I E Q D E R V Y M S Q R G D L Y F A N V E E N D S R N D Y C C F A A F P K L R T I V Q K M P M K L T V N S S N S I K Q R K P K L L L P P A Q M G S L S A K T V L K G D T L L L E C F A E G L P T P H I Q W S K P G S E L P E G R A T I E V H E K T L K I E N I S Y Q D R G N Y R C T A N N L L G K A S H D F H V T V E E P P R W K K K P Q S A V Y S T G S S G I L L C E A E G E P Q P T I K W R L N G L P I E K H P F P G D F M F P R E I S F T N L L P N H T G V Y Q C E A S N I H G T I L A N A N I D V I D V I P L I K T K N E E N Y A T V V G Y S A F L H C E Y F A S P K A T V V W E V A D E T H P L E G D R Y H T H E N G T L E I Y R T T E E D A G S Y S C W V D N A M G K A V I T A N L D I R N A T K L R V S P K N P R I P K S H V L E L Y C E S Q C D S H L K H S L K L S W S K D G E A F E M N G T E D G R I V I D G A Y L T I S N I T A E D Q G V Y S C S A Q T S L D S T S E K T Q V T V L G V P D P P G N L H L S E R Q N R S V R L S W E A G D D H N S K I S E Y I V E F E G N R E E P G K W E E L T R V Q G E E T D V V L S L A P Y V R Y Q F R V T A V N E V G R S H A S L P S D H H E T P P A A P D K N P Q N I R V Q A S Q P K E M I I K W E P L K S M E Q N G P G L E Y K V S W K P Q G A P E E W E E E I V T N H T L R V M T P T V Y A P Y D V K V Q A I N Q L G S S P D P Q P V T L Y S G E D Y P S T A P V I Q R V D V M N S T L V K V T W S S I P K E T V H G L L R G Y Q I N W W K T K S L L D G R T H P K E V N I L R F S G Q R N S G M V P S L D P F S E F H L T V L A Y N S K G A G P E S E P Y I F Q T P E G V P E Q P S F L K V I K V D K D T A T L S W G L P K K L N G N L T G Y L L Q Y Q I I N D T Y E L G E L N E I N V T T P S K S S W H L S N L N S T T K Y K F Y L R A C T S R G C G K P I S E E G A T L G E G S K G I R K I T E G V N
- Accession
- P70232
- Protein length
- Fragment
- Predicted molecular weight
- 113.9 kDa
- Amino acids
- 1 to 1027
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons as well as in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane.
- Sequence similarities
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
- Post-translational modifications
Cleavage by metalloprotease ADAM8 in the extracellular part generates 2 soluble forms (125 kDa and 165 kDa) in vitro and is inhibited by metalloprotease inhibitors. In brain extracts, these two soluble forms are also present and are dramatically reduced in mice lacking ADAM8 (PubMed:14761956). Cleaved by BACE1 (PubMed:29325091).
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target known as CHL1 also called L1CAM homolog or close homolog of L1 is a transmembrane protein involved in cell adhesion within the nervous system. It has a molecular mass of approximately 140 kDa. CHL1 is mainly expressed in the brain particularly during the development phase including neuronal cell bodies and axonal membranes. Its structural components enable it to facilitate homophilic and heterophilic interactions which are essential for neural cell communication.
- Biological function summary
CHL1 plays a critical role in neural development and synapse formation. It is part of the immunoglobulin superfamily and forms complexes with other neural cell adhesion molecules (NCAMs). CHL1 is actively involved in neurite outgrowth cell migration and axonal guidance contributing to the proper formation of neuronal networks. Its expression is tightly regulated during neural development indicating its importance in ensuring correct neural patterning.
- Pathways
CHL1 functions significantly in the axon guidance and synaptic plasticity pathways. These pathways are essential for neural differentiation and response to external stimuli. CHL1 interacts with proteins like integrins and cytoplasmic kinases such as focal adhesion kinase (FAK) modulating signal transduction processes that guide axonal development and remodeling. These interactions highlight CHL1's involvement in neural connectivity and adaptability.
- Associated diseases and disorders
CHL1 associates with psychiatric and neurodevelopmental disorders including schizophrenia and autism spectrum disorders. Altered CHL1 expression or function can lead to cognitive and behavioral deficits. Its interaction with proteins like neurofascin further emphasizes its role in maintaining neural network integrity which is often compromised in these disorders. Understanding CHL1’s interactions and functions can provide insights into therapeutic interventions for such conditions.
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SDS-PAGE - Recombinant Mouse CHL1 protein (His tag) (ab277001)
SDS-PAGE analysis of ab277001.
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