Recombinant Mouse Complement factor B protein (His tag) is a Mouse Full Length protein, in the 23 to 761 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
T P V L E A R P Q V S C S L E G V E I K G G S F Q L L Q G G Q A L E Y L C P S G F Y P Y P V Q T R T C R S T G S W S D L Q T R D Q K I V Q K A E C R A I R C P R P Q D F E N G E F W P R S P F Y N L S D Q I S F Q C Y D G Y V L R G S A N R T C Q E N G R W D G Q T A I C D D G A G Y C P N P G I P I G T R K V G S Q Y R L E D I V T Y H C S R G L V L R G S Q K R K C Q E G G S W S G T E P S C Q D S F M Y D S P Q E V A E A F L S S L T E T I E G A D A E D G H S P G E Q Q K R K I V L D P S G S M N I Y L V L D G S D S I G S S N F T G A K R C L T N L I E K V A S Y G V R P R Y G L L T Y A T V P K V L V R V S D E R S S D A D W V T E K L N Q I S Y E D H K L K S G T N T K R A L Q A V Y S M M S W A G D A P P E G W N R T R H V I I I M T D G L H N M G G N P V T V I Q D I R A L L D I G R D P K N P R E D Y L D V Y V F G V G P L V D S V N I N A L A S K K D N E H H V F K V K D M E D L E N V F Y Q M I D E T K S L S L C G M V W E H K K G N D Y H K Q P W Q A K I S V T R P L K G H E T C M G A V V S E Y F V L T A A H C F M V D D Q K H S I K V S V G G Q R R D L E I E E V L F H P K Y N I N G K K A E G I P E F Y D Y D V A L V K L K N K L K Y G Q T L R P I C L P C T E G T T R A L R L P Q T A T C K Q H K E Q L L P V K D V K A L F V S E Q G K S L T R K E V Y I K N G D K K A S C E R D A T K A Q G Y E K V K D A S E V V T P R F L C T G G V D P Y A D P N T C K G D S G G P L I V H K R S R F I Q V G V I S W G V V D V C R D Q R R Q Q L V P S Y A R D F H I N L F Q V L P W L K D K L K D E D L G F L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.
Alternative names
Complement factor B, C3/C5 convertase, Cfb, Bf, H2-Bf
Recommended products
Recombinant Mouse Complement factor B protein (His tag) is a Mouse Full Length protein, in the 23 to 761 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P04186-1
- Animal free
No
- Species
Mouse
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
T P V L E A R P Q V S C S L E G V E I K G G S F Q L L Q G G Q A L E Y L C P S G F Y P Y P V Q T R T C R S T G S W S D L Q T R D Q K I V Q K A E C R A I R C P R P Q D F E N G E F W P R S P F Y N L S D Q I S F Q C Y D G Y V L R G S A N R T C Q E N G R W D G Q T A I C D D G A G Y C P N P G I P I G T R K V G S Q Y R L E D I V T Y H C S R G L V L R G S Q K R K C Q E G G S W S G T E P S C Q D S F M Y D S P Q E V A E A F L S S L T E T I E G A D A E D G H S P G E Q Q K R K I V L D P S G S M N I Y L V L D G S D S I G S S N F T G A K R C L T N L I E K V A S Y G V R P R Y G L L T Y A T V P K V L V R V S D E R S S D A D W V T E K L N Q I S Y E D H K L K S G T N T K R A L Q A V Y S M M S W A G D A P P E G W N R T R H V I I I M T D G L H N M G G N P V T V I Q D I R A L L D I G R D P K N P R E D Y L D V Y V F G V G P L V D S V N I N A L A S K K D N E H H V F K V K D M E D L E N V F Y Q M I D E T K S L S L C G M V W E H K K G N D Y H K Q P W Q A K I S V T R P L K G H E T C M G A V V S E Y F V L T A A H C F M V D D Q K H S I K V S V G G Q R R D L E I E E V L F H P K Y N I N G K K A E G I P E F Y D Y D V A L V K L K N K L K Y G Q T L R P I C L P C T E G T T R A L R L P Q T A T C K Q H K E Q L L P V K D V K A L F V S E Q G K S L T R K E V Y I K N G D K K A S C E R D A T K A Q G Y E K V K D A S E V V T P R F L C T G G V D P Y A D P N T C K G D S G G P L I V H K R S R F I Q V G V I S W G V V D V C R D Q R R Q Q L V P S Y A R D F H I N L F Q V L P W L K D K L K D E D L G F L
- Accession
- Protein length
Full Length
- Predicted molecular weight
87 kDa
- Amino acids
23 to 761
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.
- Sequence similarities
Belongs to the peptidase S1 family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complement factor B also known as CFB protein is a component of the complement system. It plays an important role in the alternative pathway. This protein with a molecular mass of approximately 93 kDa is produced mainly in the liver and expresses in various tissues throughout the body. Factor B comprises several domains including the von Willebrand factor type A domain essential for its activity. CFB engages in protein-protein interactions important for the function of the complement cascade.
- Biological function summary
Complement factor B is essential in the immune system. It participates in the formation of the C3 convertase complex which is an important step in the amplification of the complement cascade. This protein contributes to the opsonization and clearance of pathogens assisting in the immune response. CFB binds with complement component C3b to participate in the alternative pathway which does not require antibody presence for activation.
- Pathways
Complement factor B is part of the innate immune pathway. It helps mediate the alternative complement pathway acting as an amplifier of immune responses. This pathway interacts closely with the classical and lectin pathways ensuring a versatile defense mechanism. Factor D for example also plays a complementary role by cleaving factor B when bound to C3b to form C3 convertase a critical step in propagating the immune response.
- Associated diseases and disorders
Complement factor B associates with kidney-related diseases like atypical hemolytic uremic syndrome (aHUS). Mutations in CFB can lead to deregulated complement activation contributing to pathological conditions like age-related macular degeneration (AMD). The relationship with protein C3 is important in these disorders as both factor B and C3 engage in interactions that when dysfunctional influence disease outcomes. Understanding these interactions can help develop targeted therapies such as anti-complement agents to modulate the complement system in these diseases.
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1 product image
SDS-PAGE - Recombinant Mouse Complement factor B protein (His tag) (ab226229)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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