Recombinant Mouse CRELD2 protein (His tag) is a Mouse Full Length protein, in the 1 to 350 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
M H L L L A A A F G L L L L L P P P G A V A S R K P T M C Q R C R T L V D K F N Q G M A N T A R K N F G G G N T A W E E K T L S K Y E F S E I R L L E I M E G L C D S S D F E C N Q L L E Q Q E E Q L E A W W Q T L K K E H P N L F E W F C V H T L K A C C L P G T Y G P D C Q E C Q G G S E R P C S G N G Y C S G D G S R Q G D G S C Q C H T G Y K G P L C I D C T D G F F S L Q R N E T H S I C S A C D E S C K T C S G P S N K D C I Q C E V G W A R V E D A C V D V D E C A A E T S P C S D G Q Y C E N V N G S Y T C E D C D S T C V G C T G K G P A N C K E C I A G Y T K E S G Q C T D I D E C S L E E K A C K R K N E N C Y N V P G S F V C V C P E G F E E T E D A C V Q T A E G K V T E E N P T Q P P S R E D L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protein disulfide isomerase (Probable). Might play a role in the unfolded protein response (Probable). May regulate transport of alpha4-beta2 neuronal acetylcholine receptor (By similarity).
Alternative names
Protein disulfide isomerase Creld2, Cysteine-rich with EGF-like domain protein 2, Creld2
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Recombinant Mouse CRELD2 protein (His tag) is a Mouse Full Length protein, in the 1 to 350 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Measured by the ability of the immobilized protein to support the adhesion of ATDC5 mouse chondrogenic cells. When 5 x 10^4 cells/well are added to mCRELD2-coated plates (0.625 μg/mL, 100 μL/well),approximately 20% cells will adhere after 60 minutes at 37°C.
- Accession
- Q9CYA0-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M H L L L A A A F G L L L L L P P P G A V A S R K P T M C Q R C R T L V D K F N Q G M A N T A R K N F G G G N T A W E E K T L S K Y E F S E I R L L E I M E G L C D S S D F E C N Q L L E Q Q E E Q L E A W W Q T L K K E H P N L F E W F C V H T L K A C C L P G T Y G P D C Q E C Q G G S E R P C S G N G Y C S G D G S R Q G D G S C Q C H T G Y K G P L C I D C T D G F F S L Q R N E T H S I C S A C D E S C K T C S G P S N K D C I Q C E V G W A R V E D A C V D V D E C A A E T S P C S D G Q Y C E N V N G S Y T C E D C D S T C V G C T G K G P A N C K E C I A G Y T K E S G Q C T D I D E C S L E E K A C K R K N E N C Y N V P G S F V C V C P E G F E E T E D A C V Q T A E G K V T E E N P T Q P P S R E D L
- Accession
- Q9CYA0
- Protein length
- Full Length
- Predicted molecular weight
- 37.5 kDa
- Amino acids
- 1 to 350
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Protein disulfide isomerase (Probable). Might play a role in the unfolded protein response (Probable). May regulate transport of alpha4-beta2 neuronal acetylcholine receptor (By similarity).
- Sequence similarities
Belongs to the CRELD family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cysteine-Rich with EGF-Like Domains 2 (CRELD2) is a protein encoded by the CRELD2 gene also known as Cysteine-Rich with EGF-Like Domains Protein 2. The protein consists of 362 amino acids and has a molecular mass of approximately 39 kDa. CRELD2 expresses mainly in the endoplasmic reticulum and can also be found in many tissues with notable levels in neural tissues and cardiac muscle. This protein functions as a chaperone influencing the folding and stability of other proteins within the cell.
- Biological function summary
CRELD2 plays a significant role in the endoplasmic reticulum stress response a process important for maintaining cellular homeostasis. The protein modulates cellular stress pathways and associates with several other chaperone proteins assisting in the proper folding of newly synthesized proteins. It does not associate with a particular protein complex but functions independently to ensure efficient protein synthesis and folding under stress conditions.
- Pathways
CRELD2 integrates primarily with the unfolded protein response (UPR) pathway activated during ER stress to restore normal function by halting protein translation and facilitating protein folding. In this pathway CRELD2 interacts with key proteins such as ATF6 and IRE1. These interactions enhance its role in correcting misfolded proteins and prevent the accumulation of toxic protein aggregates within cells.
- Associated diseases and disorders
CRELD2 links to several conditions especially those related to disrupted protein homeostasis like Alzheimer's disease and cardiac abnormalities. Disruptions in CRELD2 expression can impact protein folding and stress responses which contributes to the pathogenesis of these diseases. It also connects with proteins such as amyloid precursor protein (APP) and cardiac myosin emphasizing its role in neuronal and cardiac health.
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1 product image
SDS-PAGE - Recombinant Mouse CRELD2 protein (His tag) (ab276991)
SDS-PAGE analysis of ab276991
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