Skip to main content

Recombinant Mouse CSF-1-R Protein is a Mouse Fragment protein, in the 20 to 511 aa range, expressed in HEK 293, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.

Be the first to review this product! Submit a review

Key facts

Purity

HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, MS, HPLC

Biologically active

No

Amino acid sequence

A P V I E P S G P E L V V E P G E T V T L R C V S N G S V E W D G P I S P Y W T L D P E S P G S T L T T R N A T F K N T G T Y R C T E L E D P M A G S T T I H L Y V K D P A H S W N L L A Q E V T V V E G Q E A V L P C L I T D P A L K D S V S L M R E G G R Q V L R K T V Y F F S P W R G F I I R K A K V L D S N T Y V C K T M V N G R E S T S T G I W L K V N R V H P E P P Q I K L E P S K L V R I R G E A A Q I V C S A T N A E V G F N V I L K R G D T K L E I P L N S D F Q D N Y Y K K V R A L S L N A V D F Q D A G I Y S C V A S N D V G T R T A T M N F Q V V E S A Y L N L T S E Q S L L Q E V S V G D S L I L T V H A D A Y P S I Q H Y N W T Y L G P F F E D Q R K L E F I T Q R A I Y R Y T F K L F L N R V K A S E A G Q Y F L M A Q N K A G W N N L T F E L T L R Y P P E V S V T W M P V N G S D V L F C D V S G Y P Q P S V T W M E C R G H T D R C D E A Q A L Q V W N D T H P E V L S Q K P F D K V I I Q S Q L P I G T L K H N M T Y F C K T H N S V G N S S Q Y F R A V S L G Q S K Q L P D E S

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (By similarity).

Alternative names

Recommended products

Recombinant Mouse CSF-1-R Protein is a Mouse Fragment protein, in the 20 to 511 aa range, expressed in HEK 293, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.

Key facts

Purity

HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, MS, HPLC

Accession
P09581-1
Animal free

Yes

Species

Mouse

Reconstitution

Reconstitute in PBS

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

A P V I E P S G P E L V V E P G E T V T L R C V S N G S V E W D G P I S P Y W T L D P E S P G S T L T T R N A T F K N T G T Y R C T E L E D P M A G S T T I H L Y V K D P A H S W N L L A Q E V T V V E G Q E A V L P C L I T D P A L K D S V S L M R E G G R Q V L R K T V Y F F S P W R G F I I R K A K V L D S N T Y V C K T M V N G R E S T S T G I W L K V N R V H P E P P Q I K L E P S K L V R I R G E A A Q I V C S A T N A E V G F N V I L K R G D T K L E I P L N S D F Q D N Y Y K K V R A L S L N A V D F Q D A G I Y S C V A S N D V G T R T A T M N F Q V V E S A Y L N L T S E Q S L L Q E V S V G D S L I L T V H A D A Y P S I Q H Y N W T Y L G P F F E D Q R K L E F I T Q R A I Y R Y T F K L F L N R V K A S E A G Q Y F L M A Q N K A G W N N L T F E L T L R Y P P E V S V T W M P V N G S D V L F C D V S G Y P Q P S V T W M E C R G H T D R C D E A Q A L Q V W N D T H P E V L S Q K P F D K V I I Q S Q L P I G T L K H N M T Y F C K T H N S V G N S S Q Y F R A V S L G Q S K Q L P D E S

Accession

P09581

Protein length

Fragment

Predicted molecular weight

55.27 kDa

Amino acids

20 to 511

Nature

Recombinant

Specifications

Form

Lyophilized

Additional notes

>= 95 % HPLC

General info

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Post-translational modifications

Autophosphorylated in response to CSF1 or IL34 binding. Phosphorylation at Tyr-559 is important for normal down-regulation of signaling by ubiquitination, internalization and degradation. Phosphorylation at Tyr-559 and Tyr-807 is important for interaction with SRC family members, including FYN, YES1 and SRC, and for subsequent activation of these protein kinases. Phosphorylation at Tyr-697 and Tyr-921 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-721 and Tyr-807 is important for interaction with PLCG2. Phosphorylation at Tyr-974 is important for interaction with CBL. Dephosphorylation by PTPN2 negatively regulates downstream signaling and macrophage differentiation.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com