Recombinant mouse CSF-1-R protein (Fc Chimera Active) is a Mouse Fragment protein, in the 20 to 511 aa range, expressed in HEK 293, with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
A P V I E P S G P E L V V E P G E T V T L R C V S N G S V E W D G P I S P Y W T L D P E S P G S T L T T R N A T F K N T G T Y R C T E L E D P M A G S T T I H L Y V K D P A H S W N L L A Q E V T V V E G Q E A V L P C L I T D P A L K D S V S L M R E G G R Q V L R K T V Y F F S P W R G F I I R K A K V L D S N T Y V C K T M V N G R E S T S T G I W L K V N R V H P E P P Q I K L E P S K L V R I R G E A A Q I V C S A T N A E V G F N V I L K R G D T K L E I P L N S D F Q D N Y Y K K V R A L S L N A V D F Q D A G I Y S C V A S N D V G T R T A T M N F Q V V E S A Y L N L T S E Q S L L Q E V S V G D S L I L T V H A D A Y P S I Q H Y N W T Y L G P F F E D Q R K L E F I T Q R A I Y R Y T F K L F L N R V K A S E A G Q Y F L M A Q N K A G W N N L T F E L T L R Y P P E V S V T W M P V N G S D V L F C D V S G Y P Q P S V T W M E C R G H T D R C D E A Q A L Q V W N D T H P E V L S Q K P F D K V I I Q S Q L P I G T L K H N M T Y F C K T H N S V G N S S Q Y F R A V S L G Q S K Q L P D E S P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (By similarity).
Alternative names
CD115, Csfmr, Fms, Csf1r, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R
Recommended products
Recombinant mouse CSF-1-R protein (Fc Chimera Active) is a Mouse Fragment protein, in the 20 to 511 aa range, expressed in HEK 293, with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Mouse M-CSF Protein at (0.2 μg) can bind ab220450 with a linear range of 0.3-12.8 ng/mL.
- Accession
- P09581-1
- Animal free
- No
- Species
- Mouse
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: L-Arginine, Sodium chloride, 5% Trehalose, 0.75% Glycine, 0.61% Tris
Sequence info
Amino acid sequence
- A P V I E P S G P E L V V E P G E T V T L R C V S N G S V E W D G P I S P Y W T L D P E S P G S T L T T R N A T F K N T G T Y R C T E L E D P M A G S T T I H L Y V K D P A H S W N L L A Q E V T V V E G Q E A V L P C L I T D P A L K D S V S L M R E G G R Q V L R K T V Y F F S P W R G F I I R K A K V L D S N T Y V C K T M V N G R E S T S T G I W L K V N R V H P E P P Q I K L E P S K L V R I R G E A A Q I V C S A T N A E V G F N V I L K R G D T K L E I P L N S D F Q D N Y Y K K V R A L S L N A V D F Q D A G I Y S C V A S N D V G T R T A T M N F Q V V E S A Y L N L T S E Q S L L Q E V S V G D S L I L T V H A D A Y P S I Q H Y N W T Y L G P F F E D Q R K L E F I T Q R A I Y R Y T F K L F L N R V K A S E A G Q Y F L M A Q N K A G W N N L T F E L T L R Y P P E V S V T W M P V N G S D V L F C D V S G Y P Q P S V T W M E C R G H T D R C D E A Q A L Q V W N D T H P E V L S Q K P F D K V I I Q S Q L P I G T L K H N M T Y F C K T H N S V G N S S Q Y F R A V S L G Q S K Q L P D E S P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
- Accession
- P09581
- Protein length
- Fragment
- Predicted molecular weight
- 81.8 kDa
- Amino acids
- 20 to 511
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (By similarity).
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Post-translational modifications
Autophosphorylated in response to CSF1 or IL34 binding. Phosphorylation at Tyr-559 is important for normal down-regulation of signaling by ubiquitination, internalization and degradation. Phosphorylation at Tyr-559 and Tyr-807 is important for interaction with SRC family members, including FYN, YES1 and SRC, and for subsequent activation of these protein kinases. Phosphorylation at Tyr-697 and Tyr-921 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-721 and Tyr-807 is important for interaction with PLCG2. Phosphorylation at Tyr-974 is important for interaction with CBL. Dephosphorylation by PTPN2 negatively regulates downstream signaling and macrophage differentiation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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2 product images
SDS-PAGE - Recombinant mouse CSF-1-R protein (Fc Chimera Active) (ab220450)
SDS-PAGE analysis of ab220450 under reducing (lane 1) and no-reducing (lane 2) conditions, stained overnight with coomassie blue. The protein migrates as 100-130 kDa under reducing and 260 kDa under non-reducing conditions.
Functional Studies - Recombinant mouse CSF-1-R protein (Fc Chimera Active) (ab220450)
Immobilized mouse M-CSF protein at 2μg/mL (100 μL/well) can bind ab220450 with a linear range of 0.3-12.8 ng/mL.
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