Recombinant mouse CSF3 protein (Active) is a Mouse Full Length protein, in the 31 to 208 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Images
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
Amino acid sequence
V P L V T V S A L P P S L P L P R S F L L K S L E Q V R K I Q A S G S V L L E Q L C A T Y K L C H P E E L V L L G H S L G I P K A S L S G C S S Q A L Q Q T Q C L S Q L H S G L C L Y Q G L L Q A L S G I S P A L A P T L D L L Q L D V A N F A T T I W Q Q M E N L G V A P T V Q P T Q S A M P A F T S A F Q R R A G G V L A I S Y L Q G F L E T A R L A L H H L A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Additional Targets
CSF3
Alternative names
Csfg, Csf3, Granulocyte colony-stimulating factor, G-CSF
Recommended products
Recombinant mouse CSF3 protein (Active) is a Mouse Full Length protein, in the 31 to 208 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by dose dependent proliferation of M-NFS-60 cells. ED50 is ≤0.020 ng/ml, corresponding to a specific activity of 50 x 106 units/mg.
- Accession
- P09920-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- V P L V T V S A L P P S L P L P R S F L L K S L E Q V R K I Q A S G S V L L E Q L C A T Y K L C H P E E L V L L G H S L G I P K A S L S G C S S Q A L Q Q T Q C L S Q L H S G L C L Y Q G L L Q A L S G I S P A L A P T L D L L Q L D V A N F A T T I W Q Q M E N L G V A P T V Q P T Q S A M P A F T S A F Q R R A G G V L A I S Y L Q G F L E T A R L A L H H L A
- Accession
- P09920
- Protein length
- Full Length
- Predicted molecular weight
- 19 kDa
- Actual molecular weight
- 19 kDa
- Amino acids
- 31 to 208
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
- Sequence similarities
Belongs to the IL-6 superfamily.
- Post-translational modifications
O-glycosylated.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
SDS-PAGE - Recombinant mouse CSF3 protein (Active) (ab287913)
SDS-page analysis of ab287913
Mass Spectrometry - Recombinant mouse CSF3 protein (Active) (ab287913)
Mass determination by ESI-TOF.
Predicted MW is 19008.20 Da (+/- 10 Da by ESI-TOF). Observed MW is 19008.83
HPLC - Recombinant mouse CSF3 protein (Active) (ab287913)
HPLC analysis of ab287913
Functional Studies - Recombinant mouse CSF3 protein (Active) (ab287913)
Fully biologically active determined by dose dependent proliferation of M-NFS-60 cells. ED50 is ≤0.020 ng/ml, corresponding to a specific activity of 50 x 106 units/mg.
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