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AB287913

Recombinant mouse CSF3 protein (Active)

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Recombinant mouse CSF3 protein (Active) is a Mouse Full Length protein, in the 31 to 208 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

View Alternative Names

Csfg, Csf3, Granulocyte colony-stimulating factor, G-CSF

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Functional Studies - Recombinant mouse CSF3 protein (Active) (AB287913)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse CSF3 protein (Active) (AB287913)

Fully biologically active determined by dose dependent proliferation of M-NFS-60 cells. ED50 is ≤0.020 ng/ml, corresponding to a specific activity of 50 x 106 units/mg.

Mass Spectrometry - Recombinant mouse CSF3 protein (Active) (AB287913)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant mouse CSF3 protein (Active) (AB287913)

Mass determination by ESI-TOF.

Predicted MW is 19008.20 Da (+/- 10 Da by ESI-TOF). Observed MW is 19008.83

SDS-PAGE - Recombinant mouse CSF3 protein (Active) (AB287913)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse CSF3 protein (Active) (AB287913)

SDS-page analysis of ab287913

HPLC - Recombinant mouse CSF3 protein (Active) (AB287913)
  • HPLC

Supplier Data

HPLC - Recombinant mouse CSF3 protein (Active) (AB287913)

HPLC analysis of ab287913

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

HPLC, SDS-PAGE, Mass Spec, FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by dose dependent proliferation of M-NFS-60 cells. ED50 is ≤0.020 ng/ml, corresponding to a specific activity of 50 x 106 units/mg.

Accession

P09920

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"VPLVTVSALPPSLPLPRSFLLKSLEQVRKIQASGSVLLEQLCATYKLCHPEELVLLGHSLGIPKASLSGCSSQALQQTQCLSQLHSGLCLYQGLLQALSGISPALAPTLDLLQLDVANFATTIWQQMENLGVAPTVQPTQSAMPAFTSAFQRRAGGVLAISYLQGFLETARLALHHLA","proteinLength":"Full Length","predictedMolecularWeight":"19 kDa","actualMolecularWeight":"19 kDa","aminoAcidEnd":208,"aminoAcidStart":31,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P09920","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

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General info

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Sequence similarities

Belongs to the IL-6 superfamily.

Post-translational modifications

O-glycosylated.

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Product protocols

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Target data

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
See full target information Csf3
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