Recombinant Mouse CXCL10 Protein (Active)
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- What is this?
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Recombinant Mouse CXCL10 Protein (Active) is a Mouse Fragment protein, in the 22 to 98 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, Biological Activity, HPLC, FuncS.
View Alternative Names
Crg2, Ifi10, Inp10, Scyb10, Cxcl10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, C7, Interferon-gamma induced protein CRG-2, Small-inducible cytokine B10, Gamma-IP10, IP-10
- Biological Activity
Unknown
Biological Activity - Recombinant Mouse CXCL10 Protein (Active) (AB283918)
Fully biologically active determined by dose dependent cell migration of human KHYG-1 cells. ED50 for this effect is ≤3.5ng/ml corresponding to specific activity of 2.85x105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3415974 -2
- Mass Spec
Lab
Mass Spectrometry - Recombinant Mouse CXCL10 Protein (Active) (AB283918)
Mass determination by ESI-TOF.
Predicted MW is 8758.43 Da. (+/- 10 Da by ESI-TOF). Observed MW is 8757.00 Da.
Significant heterogeneity in the protein due to multiple glycoforms.
- HPLC
Lab
HPLC - Recombinant Mouse CXCL10 Protein (Active) (AB283918)
HPLC analysis of ab283918
- SDS-PAGE
Lab
SDS-PAGE - Recombinant Mouse CXCL10 Protein (Active) (AB283918)
SDS-page analysis of ab283918
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.
Pathways
The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.
Specifications
Form
Lyophilized
General info
Function
Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (By similarity) (PubMed : 28623423). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (PubMed : 18624292, PubMed : 19017990, PubMed : 28468883). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (By similarity). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (PubMed : 15456824).
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family.
Target data
Complementary Products
Product promise
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