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Recombinant Mouse CXCL10 Protein (Active) is a Mouse Fragment protein, in the 22 to 98 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, Biological Activity, HPLC.

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Images

Key facts

Purity

>95% HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, MS, Biological Activity, HPLC

Biologically active

Yes

Amino acid sequence

I P L A R T V R C N C I H I D D G P V R M R A I G K L E I I P A S L S C P R V E I I A T M K K N D E Q R C L N P E S K T I K N L M K A F S Q K R S K R A P

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Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

Biological Activity

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (By similarity) (PubMed:28623423). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (PubMed:18624292, PubMed:19017990, PubMed:28468883). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (By similarity). Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (PubMed:15456824).

Alternative names

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Recombinant Mouse CXCL10 Protein (Active) is a Mouse Fragment protein, in the 22 to 98 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, Biological Activity, HPLC.

Alternative names

Key facts

Purity

>95% HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, MS, Biological Activity, HPLC

Biological activity

Fully biologically active determined by dose dependent cell migration of human KHYG-1 cells. ED50 for this effect is ≤3.5ng/ml corresponding to specific activity of 2.85x10^5 units/mg.

Accession
P17515-1
Animal free

Yes

Species

Mouse

Reconstitution

Store lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

Concentration
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Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

I P L A R T V R C N C I H I D D G P V R M R A I G K L E I I P A S L S C P R V E I I A T M K K N D E Q R C L N P E S K T I K N L M K A F S Q K R S K R A P

Accession

P17515

Protein length

Fragment

Predicted molecular weight

8.75 kDa

Amino acids

22 to 98

Nature

Recombinant

Specifications

Form

Lyophilized

General info

Function

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (By similarity) (PubMed:28623423). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (PubMed:18624292, PubMed:19017990, PubMed:28468883). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (By similarity). Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (PubMed:15456824).

Sequence similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

Biological function summary

IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.

Activity summary

IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.

Pathways

The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.

Associated diseases and disorders

IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Biological Activity - Recombinant Mouse CXCL10 Protein (Active) (ab283918), expandable thumbnail

    Biological Activity - Recombinant Mouse CXCL10 Protein (Active) (ab283918)

    Fully biologically active determined by dose dependent cell migration of human KHYG-1 cells. ED50 for this effect is ≤3.5ng/ml corresponding to specific activity of 2.85x105 units/mg.

    Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

    Lot GR3415974 -2

  • SDS-PAGE - Recombinant Mouse CXCL10 Protein (Active) (ab283918), expandable thumbnail

    SDS-PAGE - Recombinant Mouse CXCL10 Protein (Active) (ab283918)

    SDS-page analysis of ab283918

  • Mass Spectrometry - Recombinant Mouse CXCL10 Protein (Active) (ab283918), expandable thumbnail

    Mass Spectrometry - Recombinant Mouse CXCL10 Protein (Active) (ab283918)

    Mass determination by ESI-TOF.

    Predicted MW is 8758.43 Da. (+/- 10 Da by ESI-TOF). Observed MW is 8757.00 Da.

    Significant heterogeneity in the protein due to multiple glycoforms.

  • HPLC - Recombinant Mouse CXCL10 Protein (Active) (ab283918), expandable thumbnail

    HPLC - Recombinant Mouse CXCL10 Protein (Active) (ab283918)

    HPLC analysis of ab283918

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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