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AB282382

Recombinant mouse CXCL2 protein (Active)

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Recombinant mouse CXCL2 protein (Active) is a Mouse Full Length protein, in the 28 to 100 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

View Alternative Names

Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2

4 Images
Functional Studies - Recombinant mouse CXCL2 protein (Active) (AB282382)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse CXCL2 protein (Active) (AB282382)

Fully biologically active measured by its ability to chemoattract neutrophils.

The ED50 for this effect is ≤11.97 ng/ml corresponding to specific activity of 8.35 × 104 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : ​GR3391946-1

Mass Spectrometry - Recombinant mouse CXCL2 protein (Active) (AB282382)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant mouse CXCL2 protein (Active) (AB282382)

Mass determination by ESI-TOF.

Predicted MW is 7906.36 Da. (+/- 10 Da by ESI-TOF). Observed MW is 7906.27 Da.

SDS-PAGE - Recombinant mouse CXCL2 protein (Active) (AB282382)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse CXCL2 protein (Active) (AB282382)

SDS-PAGE analysis of ab282382.

HPLC - Recombinant mouse CXCL2 protein (Active) (AB282382)
  • HPLC

Supplier Data

HPLC - Recombinant mouse CXCL2 protein (Active) (AB282382)

HPLC analysis of ab282382.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, SDS-PAGE, HPLC, FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active measured by its ability to chemoattract neutrophils.

The ED50 for this effect is ≤11.97 ng/ml corresponding to specific activity of 8.35 × 104 units/mg.

Accession

P10889

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.428% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

For research use only and are not intended for diagnostic or therapeutic use, not for use in humans.
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Sequence info

[{"sequence":"AVVASELRCQCLKTLPRVDFKNIQSLSVTPPGPHCAQTEVIATLKGGQKVCLDPEAPLVQKIIQKILNKGKAN","proteinLength":"Full Length","predictedMolecularWeight":"7.91 kDa","actualMolecularWeight":"7.91 kDa","aminoAcidEnd":100,"aminoAcidStart":28,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P10889","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CXCL2 protein also known as macrophage inflammatory protein 2 (MIP-2) or KC in the mouse model functions as a chemokine. It weighs approximately 7.8 kDa and is part of the CXC chemokine family. CXCL2 is mainly expressed in macrophages neutrophils and certain epithelial cells. It plays an important role in mediating the migration of these cells to sites of inflammation or injury.
Biological function summary

CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.

Pathways

CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).

CXCL2 is connected to inflammatory conditions and diseases like sepsis and inflammatory bowel disease (IBD). Elevated levels of CXCL2 can enhance the inflammatory response which is linked to the progression of these conditions. The protein is also associated with tumor necrosis factor-alpha (TNF-alpha) which amplifies inflammation and tissue damage during disease processes.
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Specifications

Form

Lyophilized

Additional notes

=95% purity by HPLC

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General info

Function

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.

Sequence similarities

Belongs to the intercrine alpha (chemokine CxC) family.

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Product protocols

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Target data

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.
See full target information Cxcl2
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Product promise

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