JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314622

Recombinant Mouse Cxcr2 Protein (His Tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Mouse Cxcr2 Protein (His Tag) is a Mouse Full Length protein, in the 1 to 359 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

CD182, Cmkar2, Gpcr16, Il8rb, Cxcr2, C-X-C chemokine receptor type 2, CXC-R2, CXCR-2, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL-8R B

1 Images
SDS-PAGE - Recombinant Mouse Cxcr2 Protein (His Tag) (AB314622)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse Cxcr2 Protein (His Tag) (AB314622)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P35343

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Storage buffer

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% dodecyl 2-(trimethylazaniumyl)ethyl phosphate

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGEFKVDKFNIEDFFSGDLDIFNYSSGMPSILPDAVPCHSENLEINSYAVVVIYVLVTLLSLVGNSLVMLVILYNRSTCSVTDVYLLNLAIADLFFALTLPVWAASKVNGWTFGSTLCKIFSYVKEVTFYSSVLLLACISMDRYLAIVHATSTLIQKRHLVKFVCIAMWLLSVILALPILILRNPVKVNLSTLVCYEDVGNNTSRLRVVLRILPQTFGFLVPLLIMLFCYGFTLRTLFKAHMGQKHRAMRVIFAVVLVFLLCWLPYNLVLFTDTLMRTKLIKETCERRDDIDKALNATEILGFLHSCLNPIIYAFIGQKFRHGLLKIMATYGLVSKEFLAKEGRPSFVSSSSANTSTTL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":"43.2 kDa","aminoAcidEnd":359,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P35343","tags":[{"tag":"10x His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
The product can be stored for up to 12 months
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCR2 also called C-X-C chemokine receptor type 2 is a G protein-coupled receptor with a mass of approximately 53 kDa. It primarily binds to chemokines like CXCL1 CXCL2 CXCL3 CXCL5 CXCL6 CXCL7 and CXCL8 (also known as IL-8). CXCR2 is expressed in a variety of cells but is found especially in neutrophils where it plays a role in mediating immune response. Additionally it shows expression in some epithelial and endothelial cells contributing to its specialized functions in various tissues.
Biological function summary

CXCR2 plays significant roles in mediating inflammatory responses and is part of the chemokine receptor family. It functions by promoting chemotaxis of neutrophils to sites of inflammation infection or injury. This receptor operates with other chemokine receptors in the recruitment and migration of different immune cells helping coordinate an effective immune response. CXCR2 does not function alone and often interacts with other cell surface receptors and signaling molecules during immune responses.

Pathways

CXCR2 contributes prominently to the chemokine signaling pathway and the inflammatory response pathway. It interacts closely with proteins like CXCR1 another chemokine receptor to perform its functions. These interactions can activate downstream signaling cascades involving MAPK and PI3K pathways which are important for various cellular responses like proliferation survival and migration.

CXCR2 has associations with inflammatory diseases such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In these conditions excessive activation or expression of CXCR2 can lead to pathological inflammation. CXCR2 coordinates with proteins like IL-8 contributing to the chemotaxis of inflammatory cells in affected tissues and this coordination often exacerbates the inflammatory process in these diseases.
style
left-column

Specifications

Form

Lyophilized

style
left-column

General info

Function

Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Post-translational modifications

Phosphorylated upon ligand binding; which is required for desensitization.

style
left-column

Product protocols

style
left-column

Target data

Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
See full target information Cxcr2
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB225732

Anti-CXCR2 antibody [EPR22301-103]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody [EPR22301-103] (AB225732)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB307791

Recombinant Human CD134 / OX40L receptor Protein (His-tag) (Active)

Premium Bioactive

Biological Activity - Recombinant Human CD134 / OX40L receptor Protein (His-tag) (Active) (AB307791)

  • 0
  • 0
Proteins & Peptides

AB112374

Recombinant Human Tristetraprolin/TTP protein

SDS-PAGE - Recombinant Human Tristetraprolin/TTP protein (AB112374)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com