Recombinant Mouse DHODH protein is a Mouse Full Length protein, in the 11 to 395 aa range, expressed in Cell free, with >=85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Cell free
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
L D A A I I L G G G G L L F T S Y L T A T G D D H F Y A E Y L M P A L Q R L L D P E S A H R L A V R V I S L G L L P R A T F Q D S N M L E V R V L G H K F R N P V G I A A G F D K H G E A V D G L Y K L G F G F V E V G S V T P Q P Q E G N P R P R V F R L P E D Q A V I N R Y G F N S H G L S A V E H R L R A R Q Q K Q T Q L T T D G L P L G I N L G K N K T S V D A A A D Y V E G V R I L G P L A D Y L V V N V S S P N T A G L R S L Q G K T E L R R L L S K V L Q E R D A L K G P Q K P A V L V K I A P D L T A Q D K E D I A S V A R E L G I D G L I I T N T T V S R P V G L Q G A L R S E T G G L S G K P L R D L S T Q T I R E M Y A L T Q G T I P I I G V G G V S S G Q D A L E K I Q A G A S L V Q L Y T A L T F L G P P V V A R V K R E L E A L L K E R G F N T V T D A I G V D H R R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Required for UMP biosynthesis via de novo pathway.
Alternative names
DHOdehase, Dihydroorotate oxidase, Dhodh
Recommended products
Recombinant Mouse DHODH protein is a Mouse Full Length protein, in the 11 to 395 aa range, expressed in Cell free, with >=85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Cell free
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O35435-1
- Animal free
- Yes
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% dodecyl 2-(trimethylazaniumyl)ethyl phosphate
Sequence info
Amino acid sequence
- L D A A I I L G G G G L L F T S Y L T A T G D D H F Y A E Y L M P A L Q R L L D P E S A H R L A V R V I S L G L L P R A T F Q D S N M L E V R V L G H K F R N P V G I A A G F D K H G E A V D G L Y K L G F G F V E V G S V T P Q P Q E G N P R P R V F R L P E D Q A V I N R Y G F N S H G L S A V E H R L R A R Q Q K Q T Q L T T D G L P L G I N L G K N K T S V D A A A D Y V E G V R I L G P L A D Y L V V N V S S P N T A G L R S L Q G K T E L R R L L S K V L Q E R D A L K G P Q K P A V L V K I A P D L T A Q D K E D I A S V A R E L G I D G L I I T N T T V S R P V G L Q G A L R S E T G G L S G K P L R D L S T Q T I R E M Y A L T Q G T I P I I G V G G V S S G Q D A L E K I Q A G A S L V Q L Y T A L T F L G P P V V A R V K R E L E A L L K E R G F N T V T D A I G V D H R R
- Accession
- O35435
- Protein length
- Full Length
- Predicted molecular weight
- 44.2 kDa
- Amino acids
- 11 to 395
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Required for UMP biosynthesis via de novo pathway.
- Sequence similarities
Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
- Post-translational modifications
The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
- Subcellular localisation
- Mitochondrion inner membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Dihydroorotate dehydrogenase (DHODH) also known as URA1 is an enzyme weighing approximately 43 kDa. It plays a critical role in the de novo pyrimidine biosynthesis pathway. DHODH is mainly expressed in the inner mitochondrial membrane where it catalyzes the conversion of dihydroorotate to orotate. This reaction involves ubiquinone as an electron acceptor making DHODH an important player in cellular respiration. Its functionality is essential for the synthesis of nucleotides particularly in rapidly proliferating cells.
- Biological function summary
Dihydroorotate dehydrogenase is involved in establishing the pyrimidine pool necessary for DNA and RNA synthesis. It is a single-component enzyme but its activity supports the function of other enzymes in the pathway such as carbamoyl-phosphate synthetase. The proper regulation of DHODH is integral to cellular proliferation and immune responses. Cells that synthesize nucleotides at high rates such as immune cells and cancer cells heavily depend on its activity.
- Pathways
Dihydroorotate dehydrogenase is an important player in the pyrimidine biosynthesis pathway and links to the mitochondrial electron transport chain. Its interaction with ubiquinone underlines its role in energy production connecting it to respiratory complexes. DHODH works alongside proteins such as orotate phosphoribosyltransferase which further transforms orotate to orotidine-5'-monophosphate in pyrimidine biosynthesis. These pathways are vital for maintaining the balance of nucleotide levels impacting processes like DNA replication.
- Associated diseases and disorders
Dihydroorotate dehydrogenase is relevant to autoimmune diseases like rheumatoid arthritis and cancers. Inhibitors such as leflunomide and brequinar target DHODH to disrupt the proliferation of rapidly dividing cells. Leflunomide which is metabolized to teriflunomide reduces immune cell turnover in rheumatoid arthritis illustrating DHODH's therapeutic importance. Similarly it is a target in cancer treatment where its inhibition can slow down tumor growth interfacing with other metabolic regulators active in malignancies.
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SDS-PAGE - Recombinant Mouse DHODH protein (ab289736)
SDS-PAGE analysis of ab289736
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