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AB281791

Recombinant mouse DKK1 protein (Active)

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Recombinant mouse DKK1 protein (Active) is a Mouse Full Length protein, in the 32 to 272 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for FuncS.

View Alternative Names

Dickkopf-related protein 1, Dickkopf-1, Dkk-1, mDkk-1, Dkk1

4 Images
Functional Studies - Recombinant mouse DKK1 protein (Active) (AB281791)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse DKK1 protein (Active) (AB281791)

Fully biologically active measured by its ability to inhibit Wnt3-a induced alkaline phosphatase production in C2C12 cells.

ED50 for this effect is ≤5.9 ng/ml corresponding to specific activity of 1.69 x 105 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot # GR3397988-1.

Mass Spectrometry - Recombinant mouse DKK1 protein (Active) (AB281791)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant mouse DKK1 protein (Active) (AB281791)

Mass determination by ESI-TOF.

Predicted MW is 26212.64 Da. (+/- 10 Da by ESI-TOF). Observes MW is 26213.93 Da. Additional masses are due to residual O-glycans.

SDS-PAGE - Recombinant mouse DKK1 protein (Active) (AB281791)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse DKK1 protein (Active) (AB281791)

SDS-Page anaylsis of ab281791.

HPLC - Recombinant mouse DKK1 protein (Active) (AB281791)
  • HPLC

Supplier Data

HPLC - Recombinant mouse DKK1 protein (Active) (AB281791)

HPLC analysis of ab281791.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active measured by its ability to inhibit Wnt3-a induced alkaline phosphatase production by MC3T3-E1 mouse pre-osteoblast cells.

Accession

O54908

Animal free

No

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"TLNSVLINSNAIKNLPPPLGGAGGQPGSAVSVAPGVLYEGGNKYQTLDNYQPYPCAEDEECGSDEYCSSPSRGAAGVGGVQICLACRKRRKRCMRHAMCCPGNYCKNGICMPSDHSHFPRGEIEESIIENLGNDHNAAAGDGYPRRTTLTSKIYHTKGQEGSVCLRSSDCAAGLCCARHFWSKICKPVLKEGQVCTKHKRKGSHGLEIFQRCYCGEGLACRIQKDHHQASNSSRLHTCQRH","proteinLength":"Full Length","predictedMolecularWeight":"29 kDa","actualMolecularWeight":null,"aminoAcidEnd":272,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"O54908","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.
Biological function summary

The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.

Pathways

DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.

Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.
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Specifications

Form

Lyophilized

Additional notes

>=95% Purity by HPLC

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General info

Function

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed : 18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed : 26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed : 18505822).

Sequence similarities

Belongs to the dickkopf family.

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Product protocols

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Target data

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed : 18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed : 26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed : 18505822).
See full target information Dkk1
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