Recombinant mouse DKK1 protein (Active) is a Mouse Full Length protein, in the 32 to 272 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for FuncS.
Images
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- FuncS
- Biologically active
- Yes
Amino acid sequence
T L N S V L I N S N A I K N L P P P L G G A G G Q P G S A V S V A P G V L Y E G G N K Y Q T L D N Y Q P Y P C A E D E E C G S D E Y C S S P S R G A A G V G G V Q I C L A C R K R R K R C M R H A M C C P G N Y C K N G I C M P S D H S H F P R G E I E E S I I E N L G N D H N A A A G D G Y P R R T T L T S K I Y H T K G Q E G S V C L R S S D C A A G L C C A R H F W S K I C K P V L K E G Q V C T K H K R K G S H G L E I F Q R C Y C G E G L A C R I Q K D H H Q A S N S S R L H T C Q R H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed:26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed:18505822).
Alternative names
Dickkopf-related protein 1, Dickkopf-1, Dkk-1, mDkk-1, Dkk1
Recommended products
Recombinant mouse DKK1 protein (Active) is a Mouse Full Length protein, in the 32 to 272 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for FuncS.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- FuncS
- Biologically active
- Yes
- Biological activity
- Fully biologically active measured by its ability to inhibit Wnt3-a induced alkaline phosphatase production by MC3T3-E1 mouse pre-osteoblast cells.
- Accession
- O54908-1
- Animal free
- No
- Species
- Mouse
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- T L N S V L I N S N A I K N L P P P L G G A G G Q P G S A V S V A P G V L Y E G G N K Y Q T L D N Y Q P Y P C A E D E E C G S D E Y C S S P S R G A A G V G G V Q I C L A C R K R R K R C M R H A M C C P G N Y C K N G I C M P S D H S H F P R G E I E E S I I E N L G N D H N A A A G D G Y P R R T T L T S K I Y H T K G Q E G S V C L R S S D C A A G L C C A R H F W S K I C K P V L K E G Q V C T K H K R K G S H G L E I F Q R C Y C G E G L A C R I Q K D H H Q A S N S S R L H T C Q R H
- Accession
- O54908
- Protein length
- Full Length
- Predicted molecular weight
- 29 kDa
- Amino acids
- 32 to 272
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>=95% Purity by HPLC
General info
- Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed:26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed:18505822).
- Sequence similarities
Belongs to the dickkopf family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.
- Biological function summary
The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.
- Pathways
DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.
- Associated diseases and disorders
Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.
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4 product images
Functional Studies - Recombinant mouse DKK1 protein (Active) (ab281791)
Fully biologically active measured by its ability to inhibit Wnt3-a induced alkaline phosphatase production in C2C12 cells.
ED50 for this effect is ≤5.9 ng/ml corresponding to specific activity of 1.69 x 105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot # GR3397988-1.
SDS-PAGE - Recombinant mouse DKK1 protein (Active) (ab281791)
SDS-Page anaylsis of ab281791.
HPLC - Recombinant mouse DKK1 protein (Active) (ab281791)
HPLC analysis of ab281791.
Mass Spectrometry - Recombinant mouse DKK1 protein (Active) (ab281791)
Mass determination by ESI-TOF.
Predicted MW is 26212.64 Da. (+/- 10 Da by ESI-TOF). Observes MW is 26213.93 Da. Additional masses are due to residual O-glycans.
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