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Recombinant mouse EGFR protein is a Mouse Fragment protein, in the 25 to 642 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

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Images

Functional Studies - Recombinant mouse EGFR protein (AB167752), expandable thumbnail
  • SDS-PAGE - Recombinant mouse EGFR protein (AB167752), expandable thumbnail
  • Functional Studies - Recombinant mouse EGFR protein (AB167752), expandable thumbnail
  • SDS-PAGE - Recombinant mouse EGFR protein (AB167752), expandable thumbnail

Publications

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, ELISA, FuncS

Biologically active

Yes

Amino acid sequence

L E E K K V C Q G T S N R L T Q L G T F E D H F L S L Q R M Y N N C E V V L G N L E I T Y V Q R N Y D L S F L K T I Q E V A G Y V L I A L N T V E R I P L E N L Q I I R G N A L Y E N T Y A L A I L S N Y G T N R T G L R E L P M R N L Q E I L I G A V R F S N N P I L C N M D T I Q W R D I V Q N V F M S N M S M D L Q S H P S S C P K C D P S C P N G S C W G G G E E N C Q K L T K I I C A Q Q C S H R C R G R S P S D C C H N Q C A A G C T G P R E S D C L V C Q K F Q D E A T C K D T C P P L M L Y N P T T Y Q M D V N P E G K Y S F G A T C V K K C P R N Y V V T D H G S C V R A C G P D Y Y E V E E D G I R K C K K C D G P C R K V C N G I G I G E F K D T L S I N A T N I K H F K Y C T A I S G D L H I L P V A F K G D S F T R T P P L D P R E L E I L K T V K E I T G F L L I Q A W P D N W T D L H A F E N L E I I R G R T K Q H G Q F S L A V V G L N I T S L G L R S L K E I S D G D V I I S G N R N L C Y A N T I N W K K L F G T P N Q K T K I M N N R A E K D C K A V N H V C N P L C S S E G C W G P E P R D C V S C Q N V S R G R E C V E K C N I L E G E P R E F V E N S E C I Q C H P E C L P Q A M N I T C T G R G P D N C I Q C A H Y I D G P H C V K T C P A G I M G E N N T L V W K Y A D A N N V C H L C H A N C T Y G C A G P G L Q G C E V W P S G

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

In DTT-reduced SDS-PAGE, protein migrates as 110-115 kDa.

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

13 products for Alternative Product

Target data

Function

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:8404850). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (By similarity). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (By similarity). Plays a role in enhancing learning and memory performance (PubMed:20639532). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (PubMed:35131940).

Alternative names

Recommended products

Recombinant mouse EGFR protein is a Mouse Fragment protein, in the 25 to 642 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, ELISA, FuncS

Biological activity

Active

Accession
Q01279-1
Animal free

No

Species

Mouse

Reconstitution

Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 94% PBS, 5% Trehalose

Sequence info

Amino acid sequence

L E E K K V C Q G T S N R L T Q L G T F E D H F L S L Q R M Y N N C E V V L G N L E I T Y V Q R N Y D L S F L K T I Q E V A G Y V L I A L N T V E R I P L E N L Q I I R G N A L Y E N T Y A L A I L S N Y G T N R T G L R E L P M R N L Q E I L I G A V R F S N N P I L C N M D T I Q W R D I V Q N V F M S N M S M D L Q S H P S S C P K C D P S C P N G S C W G G G E E N C Q K L T K I I C A Q Q C S H R C R G R S P S D C C H N Q C A A G C T G P R E S D C L V C Q K F Q D E A T C K D T C P P L M L Y N P T T Y Q M D V N P E G K Y S F G A T C V K K C P R N Y V V T D H G S C V R A C G P D Y Y E V E E D G I R K C K K C D G P C R K V C N G I G I G E F K D T L S I N A T N I K H F K Y C T A I S G D L H I L P V A F K G D S F T R T P P L D P R E L E I L K T V K E I T G F L L I Q A W P D N W T D L H A F E N L E I I R G R T K Q H G Q F S L A V V G L N I T S L G L R S L K E I S D G D V I I S G N R N L C Y A N T I N W K K L F G T P N Q K T K I M N N R A E K D C K A V N H V C N P L C S S E G C W G P E P R D C V S C Q N V S R G R E C V E K C N I L E G E P R E F V E N S E C I Q C H P E C L P Q A M N I T C T G R G P D N C I Q C A H Y I D G P H C V K T C P A G I M G E N N T L V W K Y A D A N N V C H L C H A N C T Y G C A G P G L Q G C E V W P S G

Accession

Q01279

Protein length

Fragment

Predicted molecular weight

70 kDa

Amino acids

25 to 642

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Lyophilized

Additional notes

ab167752 is lyophilized from 0.22 µm filtered solution.

General info

Function

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:8404850). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (By similarity). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (By similarity). Plays a role in enhancing learning and memory performance (PubMed:20639532). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (PubMed:35131940).

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

Post-translational modifications

Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitinated by OTUD7B, preventing degradation (By similarity). Ubiquitinated by RNF115 and RNF126. Ubiquitinated by ZNRF1 or CBL at different lysines in response to EGF stimulation; leading to recruitment of the ESCRT machinery and subsequent degradation in the lysosomes (By similarity). Deubiquitinated by UCHL1 leading to the inhibition of its degradation (PubMed:32494592).

Subcellular localisation

Nucleus membrane, Endosome, Endosome membrane, Nucleus

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Measured by its binding ability in a functional ELISA. Immobilized mouse EGF at 10 µg/ml can bind mouse EGFR with a linear range of 2.5–1000 ng/ml.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.

Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

Associated diseases and disorders

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.

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4 product images

  • Functional Studies - Recombinant mouse EGFR protein (ab167752), expandable thumbnail

    Functional Studies - Recombinant mouse EGFR protein (ab167752)

    Captured Mouse EGF R, His Tag on CM5 Chip via anti-His antibody can bind Mouse EGF, Fc Tag with an affinity constant of 5.44 nM as determined in SPR assay (Biacore T200).

  • SDS-PAGE - Recombinant mouse EGFR protein (ab167752), expandable thumbnail

    SDS-PAGE - Recombinant mouse EGFR protein (ab167752)

    Mouse EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Functional Studies - Recombinant mouse EGFR protein (ab167752), expandable thumbnail

    Functional Studies - Recombinant mouse EGFR protein (ab167752)

    Immobilized Mouse EGF, Fc Tag at 2 µg/mL (100 µL/well) can bind Mouse EGF R, His Tag with a linear range of 0.02-0.313 µg/mL.

  • SDS-PAGE - Recombinant mouse EGFR protein (ab167752), expandable thumbnail

    SDS-PAGE - Recombinant mouse EGFR protein (ab167752)

    SDS-PAGE analysis of reduced ab167752 stained overnight with Coomassie Blue.

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Product protocols

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