Recombinant mouse EGFR protein (Active) is a Mouse Fragment protein, in the 670 to 1210 aa range, expressed in Baculovirus infected Sf9, with >=38% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=38% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M R R R H I V R K R T L R R L L Q E R E L V E P L T P S G E A P N Q A H L R I L K E T E F K K I K V L G S G A F G T V Y K G L W I P E G E K V K I P V A I K E L R E A T S P K A N K E I L D E A Y V M A S V D N P H V C R L L G I C L T S T V Q L I T Q L M P Y G C L L D Y V R E H K D N I G S Q Y L L N W C V Q I A K G M N Y L E D R R L V H R D L A A R N V L V K T P Q H V K I T D F G L A K L L G A E E K E Y H A E G G K V P I K W M A L E S I L H R I Y T H Q S D V W S Y G V T V W E L M T F G S K P Y D G I P A S D I S S I L E K G E R L P Q P P I C T I D V Y M I M V K C W M I D A D S R P K F R E L I L E F S K M A R D P Q R Y L V I Q G D E R M H L P S P T D S N F Y R A L M D E E D M E D V V D A D E Y L I P Q Q G F F N S P S T S R T P L L S S L S A T S N N S T V A C I N R N G S C R V K E D A F L Q R Y S S D P T G A V T E D N I D D A F L P V P E Y V N Q S V P K R P A G S V Q N P V Y H N Q P L H P A P G R D L H Y Q N P H S N A V G N P E Y L N T A Q P T C L S S G F N S P A L W I Q K G S H Q M S L D N P D Y Q Q D F F P K E T K P N G I F K G P T A E N A E Y L R V A P P S S E F I G A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:8404850). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (By similarity). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (By similarity). Plays a role in enhancing learning and memory performance (PubMed:20639532). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (PubMed:35131940).
Alternative names
Epidermal growth factor receptor, Egfr
Recommended products
Recombinant mouse EGFR protein (Active) is a Mouse Fragment protein, in the 670 to 1210 aa range, expressed in Baculovirus infected Sf9, with >=38% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=38% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 49 pmole/min/mg
Assay Conditions: Assay was performed in a Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 μM ATP. The reaction to place at 30°C for 40 min. The amount of ATP transferred was calculated using Kinase reagent. - Accession
- Q01279-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.03% Sodium chloride, 0.71% Tris HCl, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M R R R H I V R K R T L R R L L Q E R E L V E P L T P S G E A P N Q A H L R I L K E T E F K K I K V L G S G A F G T V Y K G L W I P E G E K V K I P V A I K E L R E A T S P K A N K E I L D E A Y V M A S V D N P H V C R L L G I C L T S T V Q L I T Q L M P Y G C L L D Y V R E H K D N I G S Q Y L L N W C V Q I A K G M N Y L E D R R L V H R D L A A R N V L V K T P Q H V K I T D F G L A K L L G A E E K E Y H A E G G K V P I K W M A L E S I L H R I Y T H Q S D V W S Y G V T V W E L M T F G S K P Y D G I P A S D I S S I L E K G E R L P Q P P I C T I D V Y M I M V K C W M I D A D S R P K F R E L I L E F S K M A R D P Q R Y L V I Q G D E R M H L P S P T D S N F Y R A L M D E E D M E D V V D A D E Y L I P Q Q G F F N S P S T S R T P L L S S L S A T S N N S T V A C I N R N G S C R V K E D A F L Q R Y S S D P T G A V T E D N I D D A F L P V P E Y V N Q S V P K R P A G S V Q N P V Y H N Q P L H P A P G R D L H Y Q N P H S N A V G N P E Y L N T A Q P T C L S S G F N S P A L W I Q K G S H Q M S L D N P D Y Q Q D F F P K E T K P N G I F K G P T A E N A E Y L R V A P P S S E F I G A
- Accession
- Q01279
- Protein length
- Fragment
- Predicted molecular weight
- 62 kDa
- Amino acids
- 670 to 1210
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:8404850). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (By similarity). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (By similarity). Plays a role in enhancing learning and memory performance (PubMed:20639532). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (PubMed:35131940).
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
- Post-translational modifications
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitinated by OTUD7B, preventing degradation (By similarity). Ubiquitinated by RNF115 and RNF126. Ubiquitinated by ZNRF1 or CBL at different lysines in response to EGF stimulation; leading to recruitment of the ESCRT machinery and subsequent degradation in the lysosomes (By similarity). Deubiquitinated by UCHL1 leading to the inhibition of its degradation (PubMed:32494592).
- Subcellular localisation
- Nucleus membrane, Endosome, Endosome membrane, Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
- Biological function summary
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
- Pathways
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
- Associated diseases and disorders
EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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2 product images
Functional Studies - Recombinant mouse EGFR protein (Active) (ab196107)
Specific activity of ab196107 was determined to be 49 pmol/min/mg
SDS-PAGE - Recombinant mouse EGFR protein (Active) (ab196107)
10% SDS Page analysis of ab196107 (1 μg)
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