Recombinant mouse Eph receptor A6 protein (His tag) is a Mouse Fragment protein, in the 1 to 546 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M G G C E V R E F L L Q F G F F L P L L T A W T G D C S H V S N Q V V L L D T T T V M G E L G W K T Y P L N G W D A I T E M D E H N R P I H T Y Q V C N V M E P N Q N N W L R T N W I S R D A A Q K I Y V E M K F T L R D C N S I P W V L G T C K E T F N L Y Y I E S D E S H G T K F K P S Q Y I K I D T I A A D E S F T Q M D L G D R I L K L N T E I R E V G P I E R K G F Y L A F Q D I G A C I A L V S V R V F Y K K C P F T V R N L A M F P D T I P R V D S S S L V E V R G S C V K S A E E R D T P K L Y C G A D G D W L V P L G R C I C S T G Y E E I E G S C H A C R P G F Y K A F A G N T K C S K C P P H S S T Y V E A T S V C H C E K G Y F R A E K D P P S M A C T R P P S A P R N V A F N I N E T A L I L E W S P P S D T G G R K D L T Y S V I C K K C G L D T T Q C E D C G G G L R F I P R H T G L I N N S V V V L D F V S H V N Y T F E I E A M N G V S E L S I S P K P F T A I T V T T D H D A P S L I G M M R K D W A S Q N S L A L S W Q A P A F S N G A I L D Y E I K Y Y E K E H E Q L T Y S S T R S K A P S V I V T G L K P A T T Y I F H I R V R T A T G Y S G Y S Q K F E F E T G D E T S D M A A E Q G Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling (By similarity).
Alternative names
Ehk-2, Ehk2, Epha6, Ephrin type-A receptor 6, EPH homology kinase 2, EHK-2
Recommended products
Recombinant mouse Eph receptor A6 protein (His tag) is a Mouse Fragment protein, in the 1 to 546 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA.
Immobilized recombinant mouse EphA6 at 2 μg/ml (100 μl/well) can bind recombinant human EphrinA3 at a linear range of 0.31-10 ng/ml.
- Accession
- Q62413-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M G G C E V R E F L L Q F G F F L P L L T A W T G D C S H V S N Q V V L L D T T T V M G E L G W K T Y P L N G W D A I T E M D E H N R P I H T Y Q V C N V M E P N Q N N W L R T N W I S R D A A Q K I Y V E M K F T L R D C N S I P W V L G T C K E T F N L Y Y I E S D E S H G T K F K P S Q Y I K I D T I A A D E S F T Q M D L G D R I L K L N T E I R E V G P I E R K G F Y L A F Q D I G A C I A L V S V R V F Y K K C P F T V R N L A M F P D T I P R V D S S S L V E V R G S C V K S A E E R D T P K L Y C G A D G D W L V P L G R C I C S T G Y E E I E G S C H A C R P G F Y K A F A G N T K C S K C P P H S S T Y V E A T S V C H C E K G Y F R A E K D P P S M A C T R P P S A P R N V A F N I N E T A L I L E W S P P S D T G G R K D L T Y S V I C K K C G L D T T Q C E D C G G G L R F I P R H T G L I N N S V V V L D F V S H V N Y T F E I E A M N G V S E L S I S P K P F T A I T V T T D H D A P S L I G M M R K D W A S Q N S L A L S W Q A P A F S N G A I L D Y E I K Y Y E K E H E Q L T Y S S T R S K A P S V I V T G L K P A T T Y I F H I R V R T A T G Y S G Y S Q K F E F E T G D E T S D M A A E Q G Q
- Accession
- Q62413
- Protein length
- Fragment
- Predicted molecular weight
- 59.5 kDa
- Amino acids
- 1 to 546
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling (By similarity).
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Eph receptor A6 also known as EphA6 acts as a receptor tyrosine kinase which belongs to the ephrin receptor subfamily. Its structure involves a mass of approximately 110 kDa. EphA6 is expressed in various tissues with notably high expression in the brain. It plays a mechanical role by binding ephrin ligands which leads to receptor activation and downstream signaling.
- Biological function summary
Ephrin-Eph receptor interactions regulate processes like cell adhesion migration and development with EphA6 influencing neural development and plasticity. It does not form a complex on its own but interacts with ephrin ligands to initiate cellular responses. These functions are important for maintaining cellular architecture and communication during development.
- Pathways
EphA6 participates in the Ephrin signaling pathway engaging in complex interactions to mediate developmental processes. This receptor also involves itself in the neural development pathway. Proteins like EphA2 and ephrin-A1 frequently interact with EphA6 within these pathways facilitating various signaling cascades that are significant for cellular architecture.
- Associated diseases and disorders
Abnormal expression or function of EphA6 has been associated with neurodegenerative disorders like Alzheimer's disease. Changes in EphA6 participate in disruption of synaptic connections. Within cancer biology EphA6 has been implicated in tumor progression and metastasis. In these contexts it may interact with other proteins such as EGFR influencing cellular behaviors related to malignancy.
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2 product images
Functional Studies - Recombinant mouse Eph receptor A6 protein (His tag) (ab276886)
Measured by its binding ability in a functional ELISA.
Immobilized recombinant mouse EphA6 at 2 μg/ml (100 μl/well) can bind recombinant human EphrinA3 at a linear range of 0.31-10 ng/ml.
SDS-PAGE - Recombinant mouse Eph receptor A6 protein (His tag) (ab276886)
SDS-PAGE analysis of ab276886
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