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AB276751

Recombinant mouse Factor VII protein (His tag)

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Recombinant mouse Factor VII protein (His tag) is a Mouse Full Length protein, in the 1 to 446 aa range, expressed in CHO cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

Cf7, F7, Coagulation factor VII, Serum prothrombin conversion accelerator

2 Images
Functional Studies - Recombinant mouse Factor VII protein (His tag) (AB276751)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse Factor VII protein (His tag) (AB276751)

Measured by its binding ability in a functional ELISA.

Immobilized mouse F7-his at 10 μg/ml (100 μl/well) can bind biotinylated mouse F3-his.

The EC50 of biotinylated mouse F3-his is 0.1-0.3 μg/ml.

SDS-PAGE - Recombinant mouse Factor VII protein (His tag) (AB276751)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant mouse Factor VII protein (His tag) (AB276751)

SDS-PAGE analysis of ab276751

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

CHO cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA.

Immobilized mouse F7-his at 10 μg/ml (100 μl/well) can bind biotinylated mouse F3-his.

The EC50 of biotinylated mouse F3-his is 0.1-0.3 μg/ml.

Accession

P70375

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Complementary Products

Primary Antibodies

AB151543

Anti-Factor VII antibody [EP6185(2)]

RabMAb|Recombinant

  • 0
  • 0
Assay Kits

AB190810

Human Factor VII ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Factor VII ELISA Kit (AB190810)

  • 0
  • 0
Primary Antibodies

AB225497

Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-TMEM119 antibody [106-6] - Microglial marker (AB225497)

  • 0
  • 0
Primary Antibodies

AB92501

Anti-FABP4 antibody [EPR3579]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody [EPR3579] (AB92501)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MVPQAHGLLLLCFLLQLQGPLGTAVFITQEEAHGVLHRQRRANSLLEELWPGSLERECNEEQCSFEEAREIFKSPERTKQFWIVYSDGDQCASNPCQNGGTCQDHLKSYVCFCLLDFEGRNCEKSKNEQLICANENGDCDQYCRDHVGTKRTCSCHEDYTLQPDEVSCKPKVEYPCGRIPVVEKRNSSSRQGRIVGGNVCPKGECPWQAVLKINGLLLCGAVLLDARWIVTAAHCFDNIRYWGNITVVMGEHDFSEKDGDEQVRRVTQVIMPDKYIRGKINHDIALLRLHRPVTFTDYVVPLCLPEKSFSENTLARIRFSRVSGWGQLLDRGATALELMSIEVPRLMTQDCLEHAKHSSNTPKITENMFCAGYMDGTKDACKGDSGGPHATHYHGTWYLTGVVSWGEGCAAIGHIGVYTRVSQYIDWLVRHMDSKLQVGVFRLPLL","proteinLength":"Full Length","predictedMolecularWeight":"47 kDa","actualMolecularWeight":null,"aminoAcidEnd":446,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"CHO cells","accessionNumber":"P70375","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor VII also known as factor 7 or factor seven is a plasma glycoprotein with a molecular mass of approximately 50 kDa. It plays an important role in the blood coagulation process. Factor VII is mainly produced in the liver and requires vitamin K for its synthesis. In the inactive form it circulates in the plasma but upon activation to factor 7a it initiates the extrinsic pathway of blood coagulation. This activation is a critical step in hemostasis which helps to prevent excessive bleeding.
Biological function summary

Factor 7a indirectly activates further components in the blood coagulation cascade. Upon activation factor 7a becomes a part of the tissue factor (TF) complex which includes tissue factor expressed on subendothelial cells. The complex triggers the activation of factor IX and factor X propagating the coagulation cascade downstream. This series of activations amplifies the signal for clot formation highlighting factor VII's integral role in initiating coagulation.

Pathways

Factor VII is primarily involved in the extrinsic pathway of the coagulation cascade. This pathway is initiated when factor 7a forms a complex with tissue factor a protein that becomes exposed at injury sites. Factor VII is also indirectly linked to the common coagulation pathway through activation of factor X. The interaction between these pathways ensures efficient and rapid clot formation. Additionally factor IX also interacts with factor VII in the intricate network of coagulation processes that sustain hemostasis.

Deficiencies or dysfunctions in factor VII can lead to coagulopathies such as congenital factor VII deficiency which results in excessively prolonged bleeding. Conversely elevated factor VII activity can contribute to thromboembolic disorders like myocardial infarction. In these conditions factor VII often interacts with proteins like factor X and tissue factor highlighting their clinical significance in managing blood coagulation abnormalities.
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General info

Function

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa also converts factor IX to factor IXa in the presence of tissue factor and calcium (By similarity).

Sequence similarities

Belongs to the peptidase S1 family.

Post-translational modifications

The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.. Can be either O-glucosylated or O-xylosylated at Ser-93 by POGLUT1.

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Product protocols

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Target data

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa also converts factor IX to factor IXa in the presence of tissue factor and calcium (By similarity).
See full target information F7
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Product promise

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