Recombinant Mouse Fas Ligand Protein Standard (His tag)
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Recombinant Mouse Fas Ligand Protein Standard (His tag) is a Mouse Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
View Alternative Names
CD178, Apt1lg1, Cd95l, Fasl, gld, Tnfsf6, Faslg, Tumor necrosis factor ligand superfamily member 6, CD95 ligand, Fas antigen ligand, CD95-L, Fas ligand, FasL
Reactivity data
Product details
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
General info
Function
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed : 7511063). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed : 19794494, PubMed : 7532682). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (PubMed : 19794494). TNFRSF6/FAS-mediated apoptosis also has a role in the induction of peripheral tolerance (PubMed : 10779162). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (By similarity).. Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (PubMed : 19794494). Can induce apoptosis but does not appear to be essential for this process (By similarity).. FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.
Sequence similarities
Belongs to the tumor necrosis factor family.
Post-translational modifications
The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FAsL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.. Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.. N-glycosylated. Glycosylation enhances apoptotic activity.. Monoubiquitinated.
Subcellular localisation
Lysosome lumen
Target data
Product promise
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