Recombinant mouse FER protein is a Mouse Fragment protein, in the 542 to 823 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3 according to PubMed:10878010 and PubMed:19159681, but clearly plays a redundant role in STAT3 phosphorylation. According to PubMed:11134346, cells where wild type FER has been replaced by a kinase-dead mutant show no reduction in STAT3 phosphorylation. Phosphorylates TMF1. Isoform 3 lacks kinase activity.
Alternative names
Fert2, Tyrosine-protein kinase Fer, Proto-oncogene c-Fer, p94-Fer
Recommended products
Recombinant mouse FER protein is a Mouse Fragment protein, in the 542 to 823 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity: 655nmol/min/mg
- Accession
- P70451-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- Accession
- P70451
- Protein length
- Fragment
- Amino acids
- 542 to 823
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3 according to PubMed:10878010 and PubMed:19159681, but clearly plays a redundant role in STAT3 phosphorylation. According to PubMed:11134346, cells where wild type FER has been replaced by a kinase-dead mutant show no reduction in STAT3 phosphorylation. Phosphorylates TMF1. Isoform 3 lacks kinase activity.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
- Post-translational modifications
Autophosphorylated.
- Subcellular localisation
- Cytoskeleton, Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Active Poly (Glu, Tyr) peptide ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FER protein also known as mouse FER or FER-1 functions as a non-receptor tyrosine kinase within the Fes/Fps subfamily. It has a molecular mass of approximately 94 kDa. FER is expressed across various tissues notably in epithelial cells and is also present in hematopoietic and neural cells. Its kinase activity regulates cell signaling affecting processes like cell proliferation differentiation and survival.
- Biological function summary
FER acts as a regulator in signaling pathways essential for cellular communication and structure maintenance. FER interacts within cellular complexes modulating the cytoskeleton and intercellular junctions. It influences actin remodeling impacting cell adhesion and motility. FER's involvement in such activities makes it important for maintaining epithelial integrity and cellular response to external stimuli.
- Pathways
FER plays a role in the PI3K/Akt pathway and the MAPK/ERK pathway. These pathways are important for cell growth survival and proliferation. FER interacts with other proteins like STAT3 and JAK2 integrating signals that drive these pathways. By modulating key signaling events FER influences cellular functions and responses to environmental changes.
- Associated diseases and disorders
FER associates with cancer and cardiovascular diseases. Aberrant FER expression and activity have been found in various tumors linking it to cancer progression and metastasis. In cardiovascular disease FER's role in regulating endothelial function connects it to vascular abnormalities. Its interaction with proteins like VEGFR further implicates FER in the pathogenesis of these conditions serving as a potential therapeutic target.
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4 product images
Functional Studies - Recombinant mouse FER protein (ab56258)
The specific activity of FER (ab56258) was determined to be 680 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant mouse FER protein (ab56258)
SDS PAGE analysis of ab56258
Functional Studies - Recombinant mouse FER protein (ab56258)
ab56258 Kinase assay at 0.1μg/μl with Specific Activity of 655 nmol/min/mg.
SDS-PAGE - Recombinant mouse FER protein (ab56258)
ab56258 on SDS-PAGE.
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