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AB315426

Recombinant Mouse FGF-15 protein

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Recombinant Mouse FGF-15 protein is a Mouse Full Length protein, in the 26 to 218 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Fibroblast growth factor 15, FGF-15, Fgf15

1 Images
SDS-PAGE - Recombinant Mouse FGF-15 protein (AB315426)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse FGF-15 protein (AB315426)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel on purified ab315426 expressed in E.coli.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag N-Terminus SUMO tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O35622

Animal free

No

Carrier free

No

Species

Mouse

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Storage buffer

pH: 8 Constituents: 6% Trehalose, 0.158% Tris HCl, 0.029% Ethylenediaminetetraacetic acid, disodium, dihydrate

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

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Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

  • 5
  • 6
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

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Primary Antibodies

AB215206

Anti-FOXP3 antibody [EPR22102-37]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (AB215206)

  • 5
  • 5
Primary Antibodies

AB72608

Anti-GPCR TGR5 antibody

BOND RX™ Validated

Western blot - Anti-GPCR TGR5 antibody (AB72608)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL
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Sequence info

[{"sequence":"RPLAQQSQSVSDEDPLFLYGWGKITRLQYLYSAGPYVSNCFLRIRSDGSVDCEEDQNERNLLEFRAVALKTIAIKDVSSVRYLCMSADGKIYGLIRYSEEDCTFREEMDCLGYNQYRSMKHHLHIIFIQAKPREQLQDQKPSNFIPVFHRSFFETGDQLRSKMFSLPLESDSMDPFRMVEDVDHLVKSPSFQK","proteinLength":"Full Length","predictedMolecularWeight":"38.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":218,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O35622","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C|-80°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
The product can be stored for up to 12 months
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF-15 also known as fibroblast growth factor 15 is a protein with a significant role in various physiological processes. This protein belongs to the fibroblast growth factor (FGF) family and has a molecular mass of approximately 25 kDa. FGF-15 is predominantly expressed in the intestines particularly in the ileum. Its main mechanical function involves the modulation of bile acid synthesis which occurs through its actions on specific receptor targets.
Biological function summary

FGF-15 plays a critical role in liver function and metabolism. It acts as a regulatory factor in bile acid homeostasis and glucose metabolism ensuring the balance and maintenance within the body. FGF-15 does not primarily function as part of a protein complex but it exerts its influence by binding to the fibroblast growth factor receptor 4 (FGFR4) associated with the β-Klotho co-receptor eliciting a signaling cascade important for maintaining metabolic homeostasis.

Pathways

FGF-15 engages in the enterohepatic circulation of bile acids and has a pivotal role in the suppression of bile acid synthesis. One of the key pathways involves fibroblast growth factor receptor signaling pathway. In this capacity FGF-15 works in conjunction with proteins such as FGFR4 and β-Klotho forming a signaling axis that influences the expression of cytochrome P450 family 7 subfamily A member 1 (CYP7A1) an important enzyme in bile acid biosynthesis.

FGF-15 is closely associated with metabolic conditions particularly liver diseases such as non-alcoholic fatty liver disease (NAFLD). Elevated levels of protein expression or dysregulation of its pathway may contribute to the development or progression of such disorders. Additionally abnormalities in the FGF-15 signaling can also impact glucose homeostasis linking it to diabetes. In these contexts FGF-15 works closely with FGFR4 and β-Klotho both important in the therapeutic targeting of these metabolic diseases.
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Specifications

Form

Lyophilized

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General info

Function

Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression.

Sequence similarities

Belongs to the heparin-binding growth factors family.

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Product protocols

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Target data

Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression.
See full target information Fgf15
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