Recombinant Mouse FGF 23 protein (Active) is a Mouse Full Length protein, in the 25 to 251 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
Y P D T S P L L G S N W G S L T H L Y T A T A R T S Y H L Q I H R D G H V D G T P H Q T I Y S A L M I T S E D A G S V V I T G A M T R R F L C M D L H G N I F G S L H F S P E N C K F R Q W T L E N G Y D V Y L S Q K H H Y L V S L G R A K R I F Q P G T N P P P F S Q F L A R R N E V P L L H F Y T V R P R R H T R S A E D P P E R D P L N V L K P R P R A T P V P V S C S R E L P S A E E G G P A A S D P L G V L R R G R G D A R G G A G G A D R C R P F P R F V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulator of phosphate homeostasis (By similarity). Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism (By similarity). Negatively regulates osteoblasts differentiation and matrix mineralization (By similarity). Up-regulates EGR1 expression in the presence of KL (PubMed:17086194).
Alternative names
Fibroblast growth factor 23, FGF-23, Fgf23
Recommended products
Recombinant Mouse FGF 23 protein (Active) is a Mouse Full Length protein, in the 25 to 251 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent proliferation of NIH-3T3 cells in the presence of mouse klotho and heparin. ED50 is ≤ 1.26 µg/ml, corresponding to a specific activity of 7.9 x 10^2 units/mg.
- Accession
- Q9EPC2-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.428% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Y P D T S P L L G S N W G S L T H L Y T A T A R T S Y H L Q I H R D G H V D G T P H Q T I Y S A L M I T S E D A G S V V I T G A M T R R F L C M D L H G N I F G S L H F S P E N C K F R Q W T L E N G Y D V Y L S Q K H H Y L V S L G R A K R I F Q P G T N P P P F S Q F L A R R N E V P L L H F Y T V R P R R H T R S A E D P P E R D P L N V L K P R P R A T P V P V S C S R E L P S A E E G G P A A S D P L G V L R R G R G D A R G G A G G A D R C R P F P R F V
- Accession
- Q9EPC2
- Protein length
- Full Length
- Predicted molecular weight
- 25.38 kDa
- Amino acids
- 25 to 251
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Regulator of phosphate homeostasis (By similarity). Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism (By similarity). Negatively regulates osteoblasts differentiation and matrix mineralization (By similarity). Up-regulates EGR1 expression in the presence of KL (PubMed:17086194).
- Sequence similarities
Belongs to the heparin-binding growth factors family.
- Post-translational modifications
Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases (By similarity).
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
For research use only and are not intended for diagnostic or therapeutic use, not for use in humans.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Fibroblast Growth Factor 23 also known as FGF 23 is a hormone-like protein that plays a role in phosphate and vitamin D metabolism. It is a member of the fibroblast growth factor family and consists of about 251 amino acids with a mass of approximately 32 kDa. FGF 23 is predominantly expressed in bone cells especially osteocytes but it also appears in other tissues such as the thymus and brain. An important tool for quantitative measurement of FGF 23 levels is the FGF 23 ELISA kit which assists in various research and clinical settings.
- Biological function summary
FGF 23 plays an important role in regulating phosphate balance in the body. It impacts the function of kidneys by decreasing the reabsorption of phosphate and suppressing the formation of active vitamin D thereby affecting calcium regulation as well. FGF 23 does not operate alone but interacts with co-receptors such as Klotho to activate its receptor complex. The protein functions as part of a larger system that fine-tunes the balance of minerals important for maintaining bone health and metabolic functions.
- Pathways
FGF 23 is part of the bone-mineral metabolism axis and is integral to the phosphate regulation pathway. It works closely with proteins like Klotho and FGFR1 and their interaction ensures proper signaling required for maintaining serum phosphate levels. FGF 23 controls vitamin D metabolism by regulating the expression of 1-alpha-hydroxylase and 24-hydroxylase influencing the activity of the related vitamin D endocrine system. Its actions help stabilize phosphate and vitamin D status which are both essential for skeletal and mineral homeostasis.
- Associated diseases and disorders
FGF 23 has a significant connection to conditions such as Hypophosphatemic Rickets and Chronic Kidney Disease (CKD). In these cases FGF 23 levels may become dysregulated leading to abnormal phosphate metabolism and bone mineralization defects. In Hypophosphatemic Rickets altered expression of FGF 23 causes excessive phosphate wasting whereas in CKD increased FGF 23 levels are associated with cardiovascular disease risk and deteriorating kidney function. Klotho as a coreceptor for FGF 23 also influences these pathological conditions highlighting its contribution to phosphate-related disorder mechanisms.
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SDS-PAGE - Recombinant Mouse FGF 23 protein (Active) (ab282386)
SDS-PAGE analysis of ab282386.
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