Recombinant mouse FGF9/GAF protein (Active) is a Mouse Fragment protein, in the 3 to 208 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
<= 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
M P L G E V G S Y F G V Q D A V P F G N V P V L P V D S P V L L N D H L G Q S E A G G L P R G P A V T D L D H L K G I L R R R Q L Y C R T G F H L E I F P N G T I Q G T R K D H S R F G I L E F I S I A V G L V S I R G V D S G L Y L G M N E K G E L Y G S E K L T Q E C V F R E Q F E E N W Y N T Y S S N L Y K H V D T G R R Y Y V A L N K D G T P R E G T R T K R H Q K F T H F L P R P V D P D K V P E L Y K D I L S Q S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Alternative names
Fibroblast growth factor 9, FGF-9, Glia-activating factor, HBGF-9, GAF, Fgf-9, Fgf9
Recommended products
Recombinant mouse FGF9/GAF protein (Active) is a Mouse Fragment protein, in the 3 to 208 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
<= 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
3T3 proliferation ED50 ≤ 10 ng/mL (≥ 1.0 x 10^5 units/mg).
- Accession
- P54130-1
- Animal free
Yes
- Carrier free
Yes
- Species
Mouse
- Reconstitution
Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.16% Sodium phosphate
Sequence info
Amino acid sequence
M P L G E V G S Y F G V Q D A V P F G N V P V L P V D S P V L L N D H L G Q S E A G G L P R G P A V T D L D H L K G I L R R R Q L Y C R T G F H L E I F P N G T I Q G T R K D H S R F G I L E F I S I A V G L V S I R G V D S G L Y L G M N E K G E L Y G S E K L T Q E C V F R E Q F E E N W Y N T Y S S N L Y K H V D T G R R Y Y V A L N K D G T P R E G T R T K R H Q K F T H F L P R P V D P D K V P E L Y K D I L S Q S
- Accession
- Protein length
Fragment
- Predicted molecular weight
23.4 kDa
- Amino acids
3 to 208
- Nature
Recombinant
Specifications
- Form
Lyophilized
General info
- Function
Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
- Sequence similarities
Belongs to the heparin-binding growth factors family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF9 also known as GAF is a member of the fibroblast growth factor family. The FGF9 protein has a molecular mass of approximately 23 kDa. It is commonly expressed in the brain kidney and lungs during fetal development. The FGF9 protein functions as a signaling molecule influencing cell proliferation and differentiation which are essential for proper tissue formation and organogenesis.
- Biological function summary
FGF9 contributes to important processes in the development and maintenance of several tissues. It is not known to be part of a larger complex. FGF9 regulates cellular activities such as proliferation and differentiation through its interaction with specific receptors on the cell surface. Its role is significant in skeletal and nervous system development where it ensures proper maturation and function of these systems.
- Pathways
The FGF9 protein participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell survival proliferation and differentiation. FGF9 interacts closely with other fibroblast growth factors such as FGF2 to ensure effective signaling cascades. These interactions affect various cellular responses and contribute to the normal development of multiple organ systems.
- Associated diseases and disorders
Alterations in FGF9 expression can be linked to conditions such as lung fibrosis and cancer. Aberrant FGF9 signaling can contribute to uncontrolled cell proliferation and fibrosis formation. The GAF protein's dysregulation may involve interactions with receptor proteins including FGFR2 in these pathological processes. Understanding FGF9's role in these diseases helps in developing targeted therapeutic strategies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
SDS-PAGE - Recombinant mouse FGF9/GAF protein (Active) (ab238299)
Recombinant mouse FGF9 protein (Active) (ab238299) used at 1 μg.
4-20% Tris-Glycine gel, stained with Coomassie Blue.
Lane 1: Reducing conditions.
Lane 2: Non-reducing conditions.
Functional Studies - Recombinant mouse FGF9/GAF protein (Active) (ab238299)
Induced proliferation of NR6R-3T3 cells.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com