Recombinant mouse FGF9/GAF protein (Active) is a Mouse Full Length protein, in the 3 to 208 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M P L G E V G S Y F G V Q D A V P F G N V P V L P V D S P V L L N D H L G Q S E A G G L P R G P A V T D L D H L K G I L R R R Q L Y C R T G F H L E I F P N G T I Q G T R K D H S R F G I L E F I S I A V G L V S I R G V D S G L Y L G M N E K G E L Y G S E K L T Q E C V F R E Q F E E N W Y N T Y S S N L Y K H V D T G R R Y Y V A L N K D G T P R E G T R T K R H Q K F T H F L P R P V D P D K V P E L Y K D I L S Q S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Alternative names
Fgf-9, Fgf9, Fibroblast growth factor 9, FGF-9, Glia-activating factor, HBGF-9, GAF
Recommended products
Recombinant mouse FGF9/GAF protein (Active) is a Mouse Full Length protein, in the 3 to 208 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- 3T3 proliferation ED50 ≤ 10 ng/mL (≥ 1.0 x 10^5 units/mg).
- Accession
- P54130-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.16% Sodium phosphate
Sequence info
Amino acid sequence
- M P L G E V G S Y F G V Q D A V P F G N V P V L P V D S P V L L N D H L G Q S E A G G L P R G P A V T D L D H L K G I L R R R Q L Y C R T G F H L E I F P N G T I Q G T R K D H S R F G I L E F I S I A V G L V S I R G V D S G L Y L G M N E K G E L Y G S E K L T Q E C V F R E Q F E E N W Y N T Y S S N L Y K H V D T G R R Y Y V A L N K D G T P R E G T R T K R H Q K F T H F L P R P V D P D K V P E L Y K D I L S Q S
- Accession
- P54130
- Protein length
- Full Length
- Predicted molecular weight
- 23 kDa
- Amino acids
- 3 to 208
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
nan
General info
- Function
Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
- Sequence similarities
Belongs to the heparin-binding growth factors family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF9 also known as GAF is a member of the fibroblast growth factor family. The FGF9 protein has a molecular mass of approximately 23 kDa. It is commonly expressed in the brain kidney and lungs during fetal development. The FGF9 protein functions as a signaling molecule influencing cell proliferation and differentiation which are essential for proper tissue formation and organogenesis.
- Biological function summary
FGF9 contributes to important processes in the development and maintenance of several tissues. It is not known to be part of a larger complex. FGF9 regulates cellular activities such as proliferation and differentiation through its interaction with specific receptors on the cell surface. Its role is significant in skeletal and nervous system development where it ensures proper maturation and function of these systems.
- Pathways
The FGF9 protein participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell survival proliferation and differentiation. FGF9 interacts closely with other fibroblast growth factors such as FGF2 to ensure effective signaling cascades. These interactions affect various cellular responses and contribute to the normal development of multiple organ systems.
- Associated diseases and disorders
Alterations in FGF9 expression can be linked to conditions such as lung fibrosis and cancer. Aberrant FGF9 signaling can contribute to uncontrolled cell proliferation and fibrosis formation. The GAF protein's dysregulation may involve interactions with receptor proteins including FGFR2 in these pathological processes. Understanding FGF9's role in these diseases helps in developing targeted therapeutic strategies.
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2 product images
SDS-PAGE - Recombinant mouse FGF9/GAF protein (Active) (ab269182)
SDS-PAGE analysis of ab269182 at 1ug/lane under (-) non-reducing and (+) reducing conditions. 4-20% Tris glycine gel. Stained with coomassie blue.
Functional Studies - Recombinant mouse FGF9/GAF protein (Active) (ab269182)
Biological activity graph of ab269182
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