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Recombinant Mouse Fibronectin Protein Standard (His tag) is a Mouse Fragment protein, expressed in Escherichia coli, with >=80% purity and suitable for sELISA, SDS-PAGE.

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Images

SDS-PAGE - Recombinant Mouse Fibronectin Protein Standard (His tag) (AB316379), expandable thumbnail
  • Sandwich ELISA - Recombinant Mouse Fibronectin Protein Standard (His tag) (AB316379), expandable thumbnail

Key facts

Purity

>=80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

Biologically active

No

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (By similarity). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (PubMed:21768292). Participates in the regulation of type I collagen deposition by osteoblasts (PubMed:21768292). Acts as a ligand for the Lilrb4a receptor, inhibiting Fcgr1/CD64-mediated monocyte activation (PubMed:34089617).Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.AnastellinBinds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

Alternative names

Recommended products

Recombinant Mouse Fibronectin Protein Standard (His tag) is a Mouse Fragment protein, expressed in Escherichia coli, with >=80% purity and suitable for sELISA, SDS-PAGE.

Key facts

Purity

>=80% SDS-PAGE

Expression system

Escherichia coli

Applications

sELISA, SDS-PAGE

Accession
P11276-1
Animal free

Yes

Species

Mouse

Concentration
Loading...
Storage buffer

pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Sequence info

Amino acid sequence

Accession

P11276

Protein length

Fragment

Predicted molecular weight

31.2 kDa

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

General info

Function

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (By similarity). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (PubMed:21768292). Participates in the regulation of type I collagen deposition by osteoblasts (PubMed:21768292). Acts as a ligand for the Lilrb4a receptor, inhibiting Fcgr1/CD64-mediated monocyte activation (PubMed:34089617).

Post-translational modifications

Sulfated.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Recombinant Mouse Fibronectin Protein Standard (His tag) (ab316379), expandable thumbnail

    SDS-PAGE - Recombinant Mouse Fibronectin Protein Standard (His tag) (ab316379)

    SDS-PAGE analysis of ab316379

  • Sandwich ELISA - Recombinant Mouse Fibronectin Protein Standard (His tag) (ab316379), expandable thumbnail

    Sandwich ELISA - Recombinant Mouse Fibronectin Protein Standard (His tag) (ab316379)

    Sandwich ELISA with the capture antibody dilution at 2 ug/mL and detector antibody dilution at 0.5 ug/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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