Recombinant Mouse FLT1/VEGF Receptor 1 protein (Active)
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Recombinant Mouse FLT1/VEGF Receptor 1 protein (Active) is a Mouse Fragment protein, in the 23 to 759 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
View Alternative Names
Emrk2, Flt, Vegfr1, Flt1, Vascular endothelial growth factor receptor 1, VEGFR-1, Embryonic receptor kinase 2, Fms-like tyrosine kinase 1, Tyrosine-protein kinase receptor FLT, FLT-1
- Biological Activity
Supplier Data
Biological Activity - Recombinant Mouse FLT1/VEGF Receptor 1 protein (Active) (AB282387)
Loaded Human VEGF165, His Tag on Anti-His antibody Biosensor can bind Mouse VEGFR1 with an affinity constant of 0.48 nM as determined in BLI assay (GatorBio Prime).
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Mouse FLT1/VEGF Receptor 1 protein (Active) (AB282387)
SDS-PAGE analysis of ab282387.
Complementary Products
Reagents, Controls & Accessories
AB116028
Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa)
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Primary Antibodies
AB32152
Anti-VEGF Receptor 1 antibody [Y103]
RabMAb|Recombinant|20ul selling size
![Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] (AB32152)](https://content.abcam.com/products/images/vegf-receptor-1-antibody-y103-ab32152--immunoprecipitation-img3909.jpg)
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Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FLT-1 mediates important cellular responses such as cell migration and proliferation. Although it often functions in conjunction with other receptors like VEGFR-2 FLT-1 has unique binding affinities that modulate the availability of VEGF for other receptors. FLT-1 proteins as part of complex signaling networks actively contribute to maintaining vascular homeostasis and regulating blood vessel permeability.
Pathways
FLT-1 is an important component in the VEGF signaling pathway and also part of the PlGF/VEGF pathway. The receptor serves as a decoy to regulate the amount of VEGF interaction with VEGFR-2 influencing endothelial cell function and vascular permeability. By sequestering VEGF FLT-1 helps maintain the balance in this angiogenic signaling network interacting with proteins like VEGF PlGF and the anti-VEGF agents that target these pathways.
Specifications
Form
Lyophilized
General info
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression (PubMed : 30936473). May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1 (By similarity).
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Post-translational modifications
N-glycosylated.. Ubiquitinated after VEGFA-mediated autophosphorylation, leading to proteolytic degradation.. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-1169 is important for interaction with PLCG. Phosphorylation at Tyr-1213 is important for interaction with PIK3R1, PTPN11, GRB2, and PLCG. Phosphorylation at Tyr-1328 is important for endocytosis and for interaction with CBL, NCK1 and CRK. Is probably dephosphorylated by PTPRB (By similarity).
Subcellular localisation
Endosome
Target data
Product promise
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