Recombinant Mouse Frizzled 5 protein (Fc Chimera) is a Mouse Fragment protein, in the 1 to 167 aa range, expressed in CHO, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- CHO cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A R P D P S A P P S L L L L L L A Q L V G R A A A A S K A P V C Q E I T V P M C R G I G Y N L T H M P N Q F N H D T Q D E A G L E V H Q F W P L V E I H C S P D L R F F L C S M Y T P I C L P D Y H K P L P P C R S V C E R A K A G C S P L M R Q Y G F A W P E R M S C D R L P V L G G D A E V L C M D Y N R S E A T T A S P K S F P A K P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for Wnt proteins (PubMed:11092808, PubMed:18230341). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:18230341). In vitro activates WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 signaling (PubMed:11092808). In neurons, activation by WNT7A promotes formation of synapses (By similarity). May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (PubMed:11092808). Plays a role in ocular development (By similarity).
Alternative names
Frizzled-5, Fz-5, mFz5, Fzd5
Recommended products
Recombinant Mouse Frizzled 5 protein (Fc Chimera) is a Mouse Fragment protein, in the 1 to 167 aa range, expressed in CHO, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- CHO cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9EQD0-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M A R P D P S A P P S L L L L L L A Q L V G R A A A A S K A P V C Q E I T V P M C R G I G Y N L T H M P N Q F N H D T Q D E A G L E V H Q F W P L V E I H C S P D L R F F L C S M Y T P I C L P D Y H K P L P P C R S V C E R A K A G C S P L M R Q Y G F A W P E R M S C D R L P V L G G D A E V L C M D Y N R S E A T T A S P K S F P A K P
- Accession
- Q9EQD0
- Protein length
- Fragment
- Predicted molecular weight
- 42.9 kDa
- Amino acids
- 1 to 167
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor for Wnt proteins (PubMed:11092808, PubMed:18230341). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:18230341). In vitro activates WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 signaling (PubMed:11092808). In neurons, activation by WNT7A promotes formation of synapses (By similarity). May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (PubMed:11092808). Plays a role in ocular development (By similarity).
- Sequence similarities
Belongs to the G-protein coupled receptor Fz/Smo family.
- Post-translational modifications
Ubiquitinated by RNF43 and ZNRF3, leading to its degradation by the proteasome.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Frizzled 5 also known as FZD5 is a receptor that functions mechanically as a part of the Wnt signaling pathway. The Frizzled 5 protein has a molecular weight of around 75 kDa and is expressed in various tissues including the brain the eyes and the cardiovascular system. As a transmembrane receptor Frizzled 5 binds to Wnt ligands and activates intracellular signaling cascades mediating important cellular processes.
- Biological function summary
Frizzled 5 participates in cellular communication and is part of the Wnt-frizzled protein complex. This complex plays a role in embryonic development cell proliferation and cell differentiation. Through its interaction with Wnt ligands Frizzled 5 can influence cell fate determination and contribute to the maintenance of stem cells. These activities are essential for development and tissue homeostasis.
- Pathways
Knowledge of Frizzled 5 places it within both the canonical and non-canonical Wnt signaling pathways. In the canonical pathway Frizzled 5 associates with LRP5/6 co-receptors to stabilize β-catenin leading to gene transcription changes. In the non-canonical pathway it involves the activation of Ca2+ signaling. Proteins related to Frizzled 5 in these pathways include Dishevelled (DVL) and the Glycogen synthase kinase (GSK-3).
- Associated diseases and disorders
Research has linked Frizzled 5 to conditions like retinopathy of prematurity and colorectal cancer. In retinopathy Frizzled 5's interaction with Norrin—a protein related to eye vascular development—can lead to abnormal vascularization. In colorectal cancer alterations in the Wnt-Frizzled signaling are implicated with β-catenin accumulating as a consequence. The modification in these signaling pathways hints at the potential for therapeutic interventions targeting Frizzled 5.
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1 product image
SDS-PAGE - Recombinant Mouse Frizzled 5 protein (Fc Chimera) (ab276925)
SDS-PAGE analysis of ab276925
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