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AB227412

Recombinant mouse GDA protein (Active) (His tag N-Terminus)

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Recombinant mouse GDA protein (Active) (His tag N-Terminus) is a Mouse Full Length protein, in the 1 to 454 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, Mass Spec.

View Alternative Names

Guanine deaminase, Guanase, Guanine aminase, Guanine aminohydrolase, GAH, Gda

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SDS-PAGE - Recombinant mouse GDA protein (Active) (His tag N-Terminus) (AB227412)
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Supplier Data

SDS-PAGE - Recombinant mouse GDA protein (Active) (His tag N-Terminus) (AB227412)

15% SDS-PAGE analysis of 3 μg ab227412.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS, Mass Spec

applications

Biologically active

Yes

Biological activity

Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37°C.

Accession

Q9R111

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMCAARTPPLALVFRGTFVHSTWTCPMEVLRDHLLGVSDSGKIVFLEESSQQEKLAKEWCFKPCEIRELSHHEFFMPGLVDTHIHAPQYAFAGSNVDLPLLEWLNKYTFPTEQRFRSTDVAEEVYTRVVRRTLKNGTTTACYFGTIHTDSSLILAEITDKFGQRAFVGKVCMDLNDTVPEYKETTEESVKETERFVSEMLQKNYPRVKPIVTPRFTLSCTETLMSELGNIAKTHDLYIQSHISENREEIEAVKSLYPSYKNYTDVYDKNNLLTNKTVMAHGCYLSEEELNIFSERGASIAHCPNSNLSLSSGLLNVLEVLKHKVKIGLGTDVAGGYSYSMLDAIRRAVMVSNVLLINKVNEKNLTLKEVFRLATLGGSQALGLDSEIGNFEVGKEFDALLINPRASDSPIDLFYGDFVGDISEAVIQKFLYLGDDRNIEEVYVGGKQVVPFSSSV","proteinLength":"Full Length","predictedMolecularWeight":"53.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":454,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9R111","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

ab227412 was purified by conventional chromatography techniques.

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General info

Function

Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.

Sequence similarities

Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family.

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Product protocols

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Target data

Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.
See full target information Gda
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