Recombinant Mouse GFAP protein (His tag) is a Mouse Full Length protein, in the 1 to 430 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Publications
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- Journal of the Royal Society, Interface 19:202201802022Liquid biopsies for early diagnosis of brain tumours: mathematical biomarker modelling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohanna A Blee,Xia Liu,Abigail J Harland,Kavi Fatania,Stuart Currie,Kathreena M Kurian,Sabine HauertPubMed 35919979
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
M E R R R I T S A R R S Y A S E T V V R G L G P S R Q L G T M P R F S L S R M T P P L P A R V D F S L A G A L N A G F K E T R A S E R A E M M E L N D R F A S Y I E K V R F L E Q Q N K A L A A E L N Q L R A K E P T K L A D V Y Q A E L R E L R L R L D Q L T A N S A R L E V E R D N F A Q D L G T L R Q K L Q D E T N L R L E A E N N L A A Y R Q E A D E A T L A R V D L E R K V E S L E E E I Q F L R K I Y E E E V R E L R E Q L A Q Q Q V H V E M D V A K P D L T A A L R E I R T Q Y E A V A T S N M Q E T E E W Y R S K F A D L T D A A S R N A E L L R Q A K H E A N D Y R R Q L Q A L T C D L E S L R G T N E S L E R Q M R E Q E E R H A R E S A S Y Q E A L A R L E E E G Q S L K E E M A R H L Q E Y Q D L L N V K L A L D I E I A T Y R K L L E G E E N R I T I P V Q T F S N L Q I R E T S L D T K S V S E G H L K R N I V V K T V E M R D G E V I K D S K Q E H K D V V M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP, Gfap
Recommended products
Recombinant Mouse GFAP protein (His tag) is a Mouse Full Length protein, in the 1 to 430 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- P03995-1
- Animal free
No
- Species
Mouse
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
M E R R R I T S A R R S Y A S E T V V R G L G P S R Q L G T M P R F S L S R M T P P L P A R V D F S L A G A L N A G F K E T R A S E R A E M M E L N D R F A S Y I E K V R F L E Q Q N K A L A A E L N Q L R A K E P T K L A D V Y Q A E L R E L R L R L D Q L T A N S A R L E V E R D N F A Q D L G T L R Q K L Q D E T N L R L E A E N N L A A Y R Q E A D E A T L A R V D L E R K V E S L E E E I Q F L R K I Y E E E V R E L R E Q L A Q Q Q V H V E M D V A K P D L T A A L R E I R T Q Y E A V A T S N M Q E T E E W Y R S K F A D L T D A A S R N A E L L R Q A K H E A N D Y R R Q L Q A L T C D L E S L R G T N E S L E R Q M R E Q E E R H A R E S A S Y Q E A L A R L E E E G Q S L K E E M A R H L Q E Y Q D L L N V K L A L D I E I A T Y R K L L E G E E N R I T I P V Q T F S N L Q I R E T S L D T K S V S E G H L K R N I V V K T V E M R D G E V I K D S K Q E H K D V V M
- Accession
- Protein length
Full Length
- Predicted molecular weight
65.9 kDa
- Amino acids
1 to 430
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
- Sequence similarities
Belongs to the intermediate filament family.
- Post-translational modifications
Phosphorylated by PKN1.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Product promise
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3 product images
SDS-PAGE - Recombinant Mouse GFAP protein (His tag) (ab226309)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226309 with 5% enrichment gel and 15% separation gel.
Mass Spectrometry - Recombinant Mouse GFAP protein (His tag) (ab226309)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab226309 could indicate that this peptide derived from E.coli-expressed mouse GFAP.
Mass Spectrometry - Recombinant Mouse GFAP protein (His tag) (ab226309)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab226309 could indicate that this peptide derived from E.coli-expressed mouse GFAP.
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