Recombinant Mouse Glutaredoxin 1 protein (His tag) is a Mouse Full Length protein, in the 1 to 107 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A Q E F V N C K I Q S G K V V V F I K P T C P Y C R K T Q E I L S Q L P F K Q G L L E F V D I T A T N N T S A I Q D Y L Q Q L T G A R T V P R V F I G K D C I G G C S D L I S M Q Q T G E L M T R L K Q I G A L Q L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
Alternative names
Glrx1, Grx, Grx1, Glrx, Glutaredoxin-1, Thioltransferase-1, TTase-1
Recommended products
Recombinant Mouse Glutaredoxin 1 protein (His tag) is a Mouse Full Length protein, in the 1 to 107 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9QUH0-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 5
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.39% MES
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A Q E F V N C K I Q S G K V V V F I K P T C P Y C R K T Q E I L S Q L P F K Q G L L E F V D I T A T N N T S A I Q D Y L Q Q L T G A R T V P R V F I G K D C I G G C S D L I S M Q Q T G E L M T R L K Q I G A L Q L
- Accession
- Q9QUH0
- Protein length
- Full Length
- Predicted molecular weight
- 14.3 kDa
- Amino acids
- 1 to 107
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
- Sequence similarities
Belongs to the glutaredoxin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutaredoxin 1 also known as GLRX or thioltransferase is a small protein with a mass of around 12 kDa. It functions mechanically by catalyzing the reduction of disulfide bonds in proteins using glutathione as a cofactor. This enzyme plays a critical role in maintaining the redox balance within cells. Glutaredoxin 1 is widely expressed in various tissues with particularly high levels in the liver and brain. Its ubiquitous expression highlights its importance in cellular processes requiring reduction-oxidation reactions.
- Biological function summary
This enzyme acts as an important regulator in cellular redox homeostasis and defense against oxidative stress. Glutaredoxin 1 does not technically form a complex but often interacts with multiple other proteins to modulate their redox state. It is involved in the regulation of apoptosis signaling pathways and the repair of oxidatively damaged proteins. Through these actions Glutaredoxin 1 protects cells from damage and maintains proper cellular function.
- Pathways
Glutaredoxin 1 participates in key processes like the regulation of the thiol-disulfide balance and the glutathione metabolism pathway. These pathways are important for cellular detoxification and antioxidant defense. It works closely with glutathione peroxidase to mitigate oxidative damage and preserves cellular integrity by ensuring reduced forms of cysteine residues on proteins. This function links Glutaredoxin 1 to other proteins such as thioredoxin which shares similar roles in oxidative stress management.
- Associated diseases and disorders
Glutaredoxin 1 plays a significant part in neurodegenerative diseases and cardiovascular disorders. Its role in managing oxidative stress links it to conditions such as Alzheimer's disease where oxidative damage is a known contributor to disease progression. The relationship between Glutaredoxin 1 and proteins like amyloid-beta prevalent in Alzheimer's further highlights its importance. In cardiovascular disorders Glutaredoxin 1 influences the function of endothelial nitric oxide synthase a protein important for vascular health suggesting its involvement in maintaining vascular function and preventing cardiovascular diseases.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Mouse Glutaredoxin 1 protein (His tag) (ab227407)
ab227407 (3 μg) analysed by 15% SDS-PAGE.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com