Recombinant mouse GM-CSF protein (Active)

• Mass Spec

Unknown

Mass Spectrometry - Recombinant mouse GM-CSF protein (Active) (AB259385)

Mass – 1 Da (+237.23 Da : Pi[3]; + 203 Da : HexNAc[1]; +162 Da : Hex[1]) (Calc. mass 14211.77 Da).

The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water : FA (99.9 : 0.1 v/v) and 15 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) to 55 % water : FA (99.9 : 0.1 v/v) and 45 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).

• FuncS

Supplier Data

Functional Studies - Recombinant mouse GM-CSF protein (Active) (AB259385)

Fully biologically active when compared to standard. Determined by the dose dependant proliferation of MC/9 cells.

ED50 is ≤0.04 ng/mL, corresponding to a specific activity of 2.50 x 10⁷ units/mg.

• SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse GM-CSF protein (Active) (AB259385)

SDS-PAGE analysis of ab259385.

• HPLC

Supplier Data

HPLC - Recombinant mouse GM-CSF protein (Active) (AB259385)

Purity >= 95%.

The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water : TFA (99.9 : 0.1 v/v) and 20 % acetonitrile : water : TFA (90 : 9.9 : 0.1 v/v/v) to 20 % water : TFA (99.9 : 0.1 v/v) and 80 % acetonitrile : water : TFA (90 : 9.9 : 0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.