Recombinant Mouse Granulin protein (His tag) is a Mouse Full Length protein, in the 1 to 589 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M W V L M S W L A F A A G L V A G T Q C P D G Q F C P V A C C L D Q G G A N Y S C C N P L L D T W P R I T S H H L D G S C Q T H G H C P A G Y S C L L T V S G T S S C C P F S K G V S C G D G Y H C C P Q G F H C S A D G K S C F Q M S D N P L G A V Q C P G S Q F E C P D S A T C C I M V D G S W G C C P M P Q A S C C E D R V H C C P H G A S C D L V H T R C V S P T G T H T L L K K F P A Q K T N R A V S L P F S V V C P D A K T Q C P D D S T C C E L P T G K Y G C C P M P N A I C C S D H L H C C P Q D T V C D L I Q S K C L S K N Y T T D L L T K L P G Y P V K E V K C D M E V S C P E G Y T C C R L N T G A W G C C P F A K A V C C E D H I H C C P A G F Q C H T E K G T C E M G I L Q V P W M K K V I A P L R L P D P Q I L K S D T P C D D F T R C P T N N T C C K L N S G D W G C C P I P E A V C C S D N Q H C C P Q G F T C L A Q G Y C Q K G D T M V A G L E K I P A R Q T T P L Q I G D I G C D Q H T S C P V G Q T C C P S L K G S W A C C Q L P H A V C C E D R Q H C C P A G Y T C N V K A R T C E K D V D F I Q P P V L L T L G P K V G N V E C G E G H F C H D N Q T C C K D S A G V W A C C P Y L K G V C C R D G R H C C P G G F H C S A R G T K C L R K K I P R W D M F L R D P V P R P L L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed:28073925, PubMed:8496151, PubMed:28541286, PubMed:28453791, PubMed:20026663, PubMed:23041626, PubMed:27789271, PubMed:12524533). Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (PubMed:28453791, PubMed:28541286, PubMed:27789271). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed:28073925). In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (PubMed:12524533). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed:8496151). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed:23041626, PubMed:20026663).Granulin-3Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.Granulin-7Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.
Alternative names
Progranulin, PGRN, Acrogranin, Epithelin/granulin precursor, Glycoprotein of 88 Kda, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, Grn
Recommended products
Recombinant Mouse Granulin protein (His tag) is a Mouse Full Length protein, in the 1 to 589 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Alternative names
Progranulin, PGRN, Acrogranin, Epithelin/granulin precursor, Glycoprotein of 88 Kda, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, Grn
Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P28798-1
- Animal free
No
- Species
Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
M W V L M S W L A F A A G L V A G T Q C P D G Q F C P V A C C L D Q G G A N Y S C C N P L L D T W P R I T S H H L D G S C Q T H G H C P A G Y S C L L T V S G T S S C C P F S K G V S C G D G Y H C C P Q G F H C S A D G K S C F Q M S D N P L G A V Q C P G S Q F E C P D S A T C C I M V D G S W G C C P M P Q A S C C E D R V H C C P H G A S C D L V H T R C V S P T G T H T L L K K F P A Q K T N R A V S L P F S V V C P D A K T Q C P D D S T C C E L P T G K Y G C C P M P N A I C C S D H L H C C P Q D T V C D L I Q S K C L S K N Y T T D L L T K L P G Y P V K E V K C D M E V S C P E G Y T C C R L N T G A W G C C P F A K A V C C E D H I H C C P A G F Q C H T E K G T C E M G I L Q V P W M K K V I A P L R L P D P Q I L K S D T P C D D F T R C P T N N T C C K L N S G D W G C C P I P E A V C C S D N Q H C C P Q G F T C L A Q G Y C Q K G D T M V A G L E K I P A R Q T T P L Q I G D I G C D Q H T S C P V G Q T C C P S L K G S W A C C Q L P H A V C C E D R Q H C C P A G Y T C N V K A R T C E K D V D F I Q P P V L L T L G P K V G N V E C G E G H F C H D N Q T C C K D S A G V W A C C P Y L K G V C C R D G R H C C P G G F H C S A R G T K C L R K K I P R W D M F L R D P V P R P L L
- Accession
- Protein length
Full Length
- Predicted molecular weight
63 kDa
- Amino acids
1 to 589
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed:28073925, PubMed:8496151, PubMed:28541286, PubMed:28453791, PubMed:20026663, PubMed:23041626, PubMed:27789271, PubMed:12524533). Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (PubMed:28453791, PubMed:28541286, PubMed:27789271). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed:28073925). In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (PubMed:12524533). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed:8496151). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed:23041626, PubMed:20026663).
- Sequence similarities
Belongs to the granulin family.
- Post-translational modifications
N-glycosylated.
- Subcellular localisation
Lysosome
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Granulin also known as progranulin or GRN is a protein with a molecular mass of approximately 68 kDa. This protein is encoded by the GRN gene and is involved in various cellular processes. Granulin is expressed widely within the body particularly in epithelial cells immune cells and neurons. Researchers have developed methods like GRN ELISA and progranulin ELISA to quantitatively measure the levels of granulin in biological samples aiding in further understanding of its role in health and disease.
- Biological function summary
Granulin functions as a growth factor involved in tissue repair cell proliferation and inflammation. It does not function as part of a larger protein complex but acts individually in exerting its effects on cells. Granulin influences various aspects of cellular architecture and signaling pathways making it important for maintaining cellular homeostasis. Additionally the granulin protein participates in regulating networks that involve cytokines and growth factors impacting both the innate and adaptive immune responses.
- Pathways
Granulin plays significant roles in the MAPK and NF-kB signaling pathways. These pathways critically manage cellular reactions to stress inflammation and other external stimuli. Granulin modulates these processes often interacting with proteins like TNF receptors and interleukins to affect the downstream signaling events. Interactions between granulin and other signaling molecules allow it to integrate into broader networks that oversee various biological responses.
- Associated diseases and disorders
Significant links exist between granulin mutations and frontotemporal dementia (FTD) and Alzheimer's disease. Mutations in the GRN gene often lead to reduced granulin levels contributing to neurodegenerative conditions. These mutations affect the protein's normal function promoting pathological mechanisms associated with neuronal deterioration. Granulin interacts with proteins such as TDP-43 in neurodegenerative diseases where alterations might result in protein aggregation and neurotoxicity. Understanding these connections can lead to potential therapeutic interventions targeting granulin-related pathways.
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SDS-PAGE - Recombinant Mouse Granulin protein (His tag) (ab276835)
SDS-PAGE analysis of ab276835
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