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AB276754

Recombinant mouse Growth hormone receptor protein (His tag)

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Recombinant mouse Growth hormone receptor protein (His tag) is a Mouse Fragment protein, in the 1 to 273 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

Growth hormone receptor, GH receptor, Somatotropin receptor, Ghr

2 Images
Functional Studies - Recombinant mouse Growth hormone receptor protein (His tag) (AB276754)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse Growth hormone receptor protein (His tag) (AB276754)

Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone.

The ED50 for this effect is 2-8μg/mL in the presence of 50 ng/mL human growth hormone.

SDS-PAGE - Recombinant mouse Growth hormone receptor protein (His tag) (AB276754)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse Growth hormone receptor protein (His tag) (AB276754)

SDS-PAGE analysis of ab276754

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone.

The ED50 for this effect is 2-8μg/mL in the presence of 50 ng/mL human growth hormone.

Accession

P16882

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MDLCQVFLTLALAVTSSTFSGSEATPATLGKASPVLQRINPSLGTSSSGKPRFTKCRSPELETFSCYWTEGDNPDLKTPGSIQLYYAKRESQRQAARIAHEWTQEWKECPDYVSAGKNSCYFNSSYTSIWIPYCIKLTTNGDLLDQKCFTVDEIVQPDPPIGLNWTLLNISLTGIRGDIQVSWQPPPNADVLKGWIILEYEIQYKEVNESKWKVMGPIWLTYCPVYSLRMDKEHEVRVRSRQRSFEKYSEFSEVLRVIFPQTNILEACEEDIQ","proteinLength":"Fragment","predictedMolecularWeight":"30.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":273,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P16882","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Growth Hormone Receptor (GHR) also known as somatotropin receptor is a vital protein that mediates the effects of growth hormone (GH). GHR is a transmembrane receptor with a mass of approximately 70 kDa and is expressed in many tissues including liver muscle and adipose tissue. It plays an important role in growth regulation by binding to growth hormone which triggers signal transduction pathways involved in cellular proliferation and growth.
Biological function summary

The growth hormone receptor facilitates the hormone's ability to promote growth and cell differentiation. The receptor when activated stimulates the JAK2/STAT5 signaling pathway a part of a larger GHR complex. This complex leads to gene transcription that stimulates growth and metabolism. The activation of GHR has significant effects on the cellular functions related to protein synthesis lipid metabolism and glucose metabolism.

Pathways

The growth hormone receptor is an important component of the JAK-STAT signaling pathway and the mTOR signaling pathway. In these pathways GHR interacts with other proteins such as Janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5). These interactions play a critical role in translating the binding of growth hormone into cellular responses that lead to growth and metabolic regulation.

Disruptions or mutations in the growth hormone receptor can lead to conditions such as Laron syndrome and growth hormone insensitivity. These conditions often display symptoms of dwarfism and metabolic aberrations due to impaired signaling. GHR malfunction relates closely to growth hormone (GH) and anti-growth hormone treatments like somatropin used for managing growth deficiencies. The connection of GHR with related pathways and proteins like the IGF-1 (Insulin-like Growth Factor 1) underlines its role in metabolic diseases.
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Specifications

Form

Lyophilized

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General info

Function

Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway.. Growth hormone-binding protein. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 1 subfamily.

Post-translational modifications

The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE (By similarity). Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17 (By similarity).. On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2.. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome (PubMed:21980433). Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of growth hormone receptor signaling (PubMed:21980433). Ubiquitination is not sufficient for GHR internalization (By similarity).

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Product protocols

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Target data

Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway.. Growth hormone-binding protein. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
See full target information Ghr
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