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AB277010

Recombinant Mouse HEXB protein (His tag)

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Recombinant Mouse HEXB protein (His tag) is a Mouse Full Length protein, in the 1 to 536 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Beta-hexosaminidase subunit beta, Beta-N-acetylhexosaminidase subunit beta, N-acetyl-beta-glucosaminidase subunit beta, Hexosaminidase subunit B, Hexb

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SDS-PAGE - Recombinant Mouse HEXB protein (His tag) (AB277010)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse HEXB protein (His tag) (AB277010)

SDS-PAGE analysis of ab277010

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB116028

Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (AB116028)

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Primary Antibodies

AB140649

Anti-HEXB antibody [EPR7978]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] (AB140649)

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  • 0
Primary Antibodies

AB252543

Anti-PICK1 antibody [L20/8]

Recombinant|KO Validated

Western blot - Anti-PICK1 antibody [L20/8] (AB252543)

  • 0
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Assay Kits

AB150674

Iron Stain Kit (Prussian Blue Stain)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Iron Stain Kit (Prussian Blue Stain) (AB150674)

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Assay Kits

AB150662

Alcian Blue Stain Kit (pH 2.5, Mucin Stain)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (AB150662)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MPQSPRSAPGLLLLQALVSLVSLALVAPARLQPALWPFPRSVQMFPRLLYISAEDFSIDHSPNSTAGPSCSLLQEAFRRYYNYVFGFYKRHHGPARFRAEPQLQKLLVSITLESECESFPSLSSDETYSLLVQEPVAVLKANSVWGALRGLETFSQLVYQDSFGTFTINESSIADSPRFPHRGILIDTSRHFLPVKTILKTLDAMAFNKFNVLHWHIVDDQSFPYQSTTFPELSNKGSYSLSHVYTPNDVRMVLEYARLRGIRVIPEFDTPGHTQSWGKGQKNLLTPCYNQKTKTQVFGPVDPTVNTTYAFFNTFFKEISSVFPDQFIHLGGDEVEFQCWASNPNIQGFMKRKGFGSDFRRLESFYIKKILEIISSLKKNSIVWQEVFDDKVELQPGTVVEVWKSEHYSYELKQVTGSGFPAILSAPWYLDLISYGQDWKNYYKVEPLNFEGSEKQKQLVIGGEACLWGEFVDATNLTPRLWPRASAVGERLWSPKTVTDLENAYKRLAVHRCRMVSRGIAAQPLYTGYCNYENKI","proteinLength":"Full Length","predictedMolecularWeight":"59.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":536,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P20060","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target known as HEXB also called Hexosaminidase subunit beta consists of protein subunit that helps in the formation of the enzyme beta-hexosaminidase. This enzyme is active in lysosomes and it weighs about 63 kDa. Beta-hexosaminidase functions to break down GM2 gangliosides which are molecules in cell membranes. HEXB expresses mainly in the brain and liver tissues signaling its role in neurological and hepatic processes.
Biological function summary

The beta-hexosaminidase enzyme formed by HEXB participates in the lysosomal degradation pathway. This enzyme requires formation of a complex with its counterpart HEXA to become biologically active. Together they catalyze the hydrolysis of GM2 gangliosides into GM3 gangliosides which is an essential step for cell membrane metabolism. This process is important for maintaining cellular homeostasis and preventing accumulation of toxic substances within cells.

Pathways

The HEXB encoded enzyme participates in sphingolipid metabolism an integral component of lipid metabolic pathways. Sphingolipids are part of many cellular functions including signal transmission and cell recognition. HEXB through the lysosomal degradation of gangliosides works closely with HEXA and NEU1 which further processes GM3 gangliosides into simpler molecules. Such interactions underline HEXB’s significant role in maintaining the balance of lipid metabolism within cells.

Mutations or deficiencies in HEXB lead to serious genetic conditions such as Sandhoff disease. This disorder results from the improper breakdown of gangliosides due to the lack of functional HEXB leading to their accumulation which severely damages nerve cells and results in neurological impairments. The partnership between HEXB and HEXA emphasizes the importance of both subunits as malfunction in either can contribute to disease progression by disrupting normal ganglioside metabolism.
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General info

Function

Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida (PubMed : 8253842).

Sequence similarities

Belongs to the glycosyl hydrolase 20 family.

Subcellular localisation

Lysosome

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Product protocols

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Target data

Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida (PubMed : 8253842).
See full target information Hexb
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