JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB322281

Recombinant Mouse ICAM 1 Protein Standard (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Mouse ICAM 1 Protein Standard (His tag) is a Mouse Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.

View Alternative Names

CD54, Intercellular adhesion molecule 1, ICAM-1, MALA-2, MyD10, Icam1

2 Images
Sandwich ELISA - Recombinant Mouse ICAM 1 Protein Standard (His tag) (AB322281)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Mouse ICAM 1 Protein Standard (His tag) (AB322281)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Mouse ICAM 1 Protein Standard (His tag) (AB322281)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse ICAM 1 Protein Standard (His tag) (AB322281)

SDS-PAGE analysis of ab322281 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

P13597

Animal free

Yes

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

style
left-column

Complementary Products

Proteins & Peptides

AB283920

Recombinant human CD28 protein

Mass Spectrometry - Recombinant human CD28 protein (AB283920)

  • 0
  • 0
Proteins & Peptides

AB288813

Recombinant Human CD80 protein (Active)

Premium Bioactive

Biological Activity - Recombinant Human CD80 protein (Active) (AB288813)

  • 0
  • 0
Proteins & Peptides

AB259398

Recombinant human EGF protein (Active)

Premium Bioactive

Functional Studies - Recombinant human EGF protein (Active) (AB259398)

  • 5
  • 2
Proteins & Peptides

AB282385

Recombinant Human EGFR protein

SDS-PAGE - Recombinant Human EGFR protein (AB282385)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.

style
left-column

Sequence info

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"51.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P13597","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength. Also functions as a ligand receptor of the signaling receptor ITGAM : ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells. During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Promotes cell aggregation in epithelial cells through interaction with MUC1.

Sequence similarities

Belongs to the immunoglobulin superfamily. ICAM family.

Post-translational modifications

Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

style
left-column

Product protocols

style
left-column

Target data

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength. Also functions as a ligand receptor of the signaling receptor ITGAM : ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells. During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Promotes cell aggregation in epithelial cells through interaction with MUC1.
See full target information Icam1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com