Recombinant mouse ICAM1 protein (Active) is a Mouse Fragment protein, in the 28 to 221 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
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Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC
- Biologically active
Yes
Amino acid sequence
Q V S I H P R E A F L P Q G G S V Q V N C S S S C K E D L S L G L E T Q W L K D E L E S G P N W K L F E L S E I G E D S S P L C F E N C G T V Q S S A S A T I T V Y S F P E S V E L R P L P A W Q Q V G K D L T L R C H V D G G A P R T Q L S A V L L R G E E I L S R Q P V G G H P K D P K E I T F T V L A S R G D H G A N F S C R T E L D L R P Q G L A L F S N V S E A R S L R T F D L P A T I P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
Alternative names
Intercellular adhesion molecule 1, ICAM-1, MALA-2, MyD10, Icam-1, Icam1
Recommended products
Recombinant mouse ICAM1 protein (Active) is a Mouse Fragment protein, in the 28 to 221 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC
- Biologically active
Yes
- Biological activity
Addition of PMA-induced HSB-2 cells to mouse ICAM1 coated plates (12.5 μg/mL, 100 μL/well) led to 40.75% cell adherence specifically. Statistical analysis was performed using two-tailed unpaired t-test, **=p<0.01.
- Accession
- P13597-1
- Animal free
Yes
- Species
Mouse
- Reconstitution
Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
Q V S I H P R E A F L P Q G G S V Q V N C S S S C K E D L S L G L E T Q W L K D E L E S G P N W K L F E L S E I G E D S S P L C F E N C G T V Q S S A S A T I T V Y S F P E S V E L R P L P A W Q Q V G K D L T L R C H V D G G A P R T Q L S A V L L R G E E I L S R Q P V G G H P K D P K E I T F T V L A S R G D H G A N F S C R T E L D L R P Q G L A L F S N V S E A R S L R T F D L P A T I P
- Accession
- Protein length
Fragment
- Predicted molecular weight
21.19 kDa
- Actual molecular weight
21.2 kDa
- Amino acids
28 to 221
- Nature
Recombinant
Specifications
- Form
Lyophilized
- Additional notes
=95% Purity by HPLC
General info
- Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
- Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
- Post-translational modifications
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
Ambient
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
SDS-PAGE - Recombinant mouse ICAM1 protein (Active) (ab277758)
SDS-PAGE analysis of ab277758.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3373514-1
Mass Spectrometry - Recombinant mouse ICAM1 protein (Active) (ab277758)
Mass determination by ESI-TOF.
Predicted MW is 21191.80 Da (+/- 10 Da by ESI-TOF). Observed MW is 21195.45 Da. Additional masses at 21357.41 and 21519.53 are due to O-linked glycosylation.
HPLC - Recombinant mouse ICAM1 protein (Active) (ab277758)
HPLC analysis of ab277758.
Functional Studies - Recombinant mouse ICAM1 protein (Active) (ab277758)
Addition of PMA-induced HSB-2 cells to mouse ICAM1 coated plates (12.5 μg/mL, 100 μL/well) led to 40.75% specific cell adherence. Statistical analysis was performed using two-tailed unpaired t-test, **=p<0.01.
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