JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB214990

Recombinant mouse ICOS Ligand/ICOSL protein (Active)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant mouse ICOS Ligand/ICOSL protein (Active) is a Mouse Fragment protein, in the 48 to 279 aa range, expressed in CHO cells, with >98%, < 0.06 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

CD275, B7h, B7h2, B7rp1, Icosl, Icoslg, ICOS ligand, B7 homolog 2, B7-like protein Gl50, B7-related protein 1, LICOS, B7-H2, B7RP-1

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 0.06 EU/µg

Expression system

CHO cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized human ICOS at 1μg/ml (100μl/well) can bind biotinylated B7-H2 (mouse):Fc (mouse) (rec.) (non-lytic) with a linear range of 0.1-1.0μg/ml. Optimal dilutions should be determined by each laboratory for each application.

Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcγR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Accession

Q9JHJ8

Animal free

No

Carrier free

Yes

Species

Mouse

Reconstitution

Reconstitute at 100 µg/mL in PBS

Storage buffer

Constituents: PBS

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product was previously labelled as ICOS Ligand
style
left-column

Sequence info

[{"sequence":"TEVGAMVGSNVVLSCIDPHRRHFNLSGLYVYWQIENPEVSVTYYLPYKSPGINVDSSYKNRGHLSLDSMKQGNFSLYLKNVTPQDTQEFTCRVFMNTATELVKILEEVVRLRVAANFSTPVISTSDSSNPGQERTYTCMSKNGYPEPNLYWINTTDNSLIDTALQNNTVYLNKLGLYDVISTLRLPWTSRGDVLCCVENVALHQNITSISQAESFTGNNTKNPQETHNNELK","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":279,"aminoAcidStart":48,"nature":"Recombinant","expressionSystem":"CHO cells","accessionNumber":"Q9JHJ8","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ICOS Ligand also known as ICOSL B7-H2 and HK53 is a critical component in immune system function. This protein with a molecular mass of approximately 65 kDa is expressed on professional antigen-presenting cells such as dendritic cells macrophages and B cells. It plays a significant mechanical role in the immune response by engaging with its counter receptor the ICOS protein present on the surface of activated T cells. This interaction supports the regulation of T cell activity influencing proliferation and cytokine production.
Biological function summary

ICOSL contributes to the modulation of immune responses. By forming a complex with the active ICOS protein ICOSL is involved in the co-stimulation of T cells. This process is important for the development of T-cell-dependent immune responses and helps in promoting the survival and differentiation of T cells. Together the ICOS ligand and ICOS protein create a dynamic interaction that balances immune activation and tolerance which is essential for maintaining immune homeostasis.

Pathways

The ICOS-ICOSL interaction plays an important role in the T-cell receptor (TCR) signaling pathway. This pathway is fundamental for the activation of T cells and subsequent immune response. ICOSL also connects to the PI3K/Akt pathway where it impacts cell survival and proliferation. ICOS and the PI3K/Akt pathway work together to influence various aspects of immune regulation and adaptive immunity.

ICOSL has a notable impact on autoimmune diseases and cancer. Its function in immune modulation when dysregulated can contribute to autoimmune conditions such as systemic lupus erythematosus. In this context the abnormal activation of the ICOS protein may exaggerate immune responses leading to pathology. In cancer high expression of ICOSL can support tumor progression by affecting the immune surveillance capability. These associations reveal ICOSL’s role in balancing immune activation and inhibition which is important in the context of disease.
style
left-column

Specifications

Form

Lyophilized

style
left-column

General info

Function

Ligand for the T-cell-specific cell surface receptor ICOS (PubMed : 10549624, PubMed : 10617205, PubMed : 10657606). Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells (PubMed : 10549624, PubMed : 10617205, PubMed : 10657606). Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (Probable). During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.

Sequence similarities

Belongs to the immunoglobulin superfamily. BTN/MOG family.

style
left-column

Product protocols

style
left-column

Target data

Ligand for the T-cell-specific cell surface receptor ICOS (PubMed : 10549624, PubMed : 10617205, PubMed : 10657606). Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells (PubMed : 10549624, PubMed : 10617205, PubMed : 10657606). Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (Probable). During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.
See full target information Icoslg
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB163782

Recombinant Human VISTA protein

SDS-PAGE - Recombinant Human VISTA protein (AB163782)

  • 0
  • 0
Proteins & Peptides

AB288813

Recombinant Human CD80 protein (Active)

Premium Bioactive

Biological Activity - Recombinant Human CD80 protein (Active) (AB288813)

  • 0
  • 0
Proteins & Peptides

AB161322

Recombinant Human HHLA2 protein

SDS-PAGE - Recombinant Human HHLA2 protein (AB161322)

  • 0
  • 0
Proteins & Peptides

AB283481

Recombinant Human PD-L2 protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant Human PD-L2 protein (Active) (AB283481)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com