Recombinant mouse IFNGR1 protein (His tag) is a Mouse Fragment protein, in the 1 to 253 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M G P Q A A A G R M I L L V V L M L S A K V G S G A L T S T E D P E P P S V P V P T N V L I K S Y N L N P V V C W E Y Q N M S Q T P I F T V Q V K V Y S G S W T D S C T N I S D H C C N I Y E Q I M Y P D V S A W A R V K A K V G Q K E S D Y A R S K E F L M C L K G K V G P P G L E I R R K K E E Q L S V L V F H P E V V V N G E S Q G T M F G D G S T C Y T F D Y T V Y V E H N R S G E I L H T K H T V E K E E C N E T L C E L N I S V S T L D S R Y C I S V D G I S S F W Q V R T E K S K D V C I P P F H D D R K D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (, PubMed:20926559, PubMed:27286456). Associates with transmembrane accessory factor IFNGR2 to form a functional receptor (PubMed:2137461, PubMed:2530216, PubMed:2530582, PubMed:2531896, PubMed:2532365). Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to its dimerization, translocation to the nucleus, and stimulation of target gene transcription (PubMed:19889125). STAT3 can also be activated in a similar manner although activation seems weaker (PubMed:15284232). IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1 (PubMed:15522878).
Alternative names
CD119, Ifngr, Ifngr1, Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha
Recommended products
Recombinant mouse IFNGR1 protein (His tag) is a Mouse Fragment protein, in the 1 to 253 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its ability to bind with recombinant mouse IFNG-Fc in a functional ELISA.
- Accession
- P15261-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M G P Q A A A G R M I L L V V L M L S A K V G S G A L T S T E D P E P P S V P V P T N V L I K S Y N L N P V V C W E Y Q N M S Q T P I F T V Q V K V Y S G S W T D S C T N I S D H C C N I Y E Q I M Y P D V S A W A R V K A K V G Q K E S D Y A R S K E F L M C L K G K V G P P G L E I R R K K E E Q L S V L V F H P E V V V N G E S Q G T M F G D G S T C Y T F D Y T V Y V E H N R S G E I L H T K H T V E K E E C N E T L C E L N I S V S T L D S R Y C I S V D G I S S F W Q V R T E K S K D V C I P P F H D D R K D
- Accession
- P15261
- Protein length
- Fragment
- Predicted molecular weight
- 27.4 kDa
- Amino acids
- 1 to 253
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (, PubMed:20926559, PubMed:27286456). Associates with transmembrane accessory factor IFNGR2 to form a functional receptor (PubMed:2137461, PubMed:2530216, PubMed:2530582, PubMed:2531896, PubMed:2532365). Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to its dimerization, translocation to the nucleus, and stimulation of target gene transcription (PubMed:19889125). STAT3 can also be activated in a similar manner although activation seems weaker (PubMed:15284232). IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1 (PubMed:15522878).
- Sequence similarities
Belongs to the type II cytokine receptor family.
- Post-translational modifications
Phosphorylated at Ser/Thr residues. Phosphorylation of Tyr-445 is required for IFNG receptor signal transduction. Influenza virus infection leads to phosphorylation in a CSNK1A1-dependent manner.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IFNGR1 protein also known as Interferon Gamma Receptor 1 is an integral component of the cell surface receptor for interferon-gamma (IFN-γ). It possesses a mass of approximately 53 kDa. IFNGR1 is expressed in various cell types including immune cells such as T cells and macrophages and is also present in other cell lines like HEK 293. This receptor binds to its specific ligand IFN-γ initiating a series of intracellular events that are essential for immune response modulation.
- Biological function summary
IFNGR1 functions as part of the interferon-gamma receptor complex working alongside the IFNGR2 subunit. This interaction with IFNGR2 is important for the receptor to properly transmit signals inside the cell. The binding of interferon-gamma to this receptor complex activates the JAK-STAT signaling pathway promoting the transcription of genes that enhance the antimicrobial activity of immune cells and regulate cellular immunity.
- Pathways
The IFNGR1 protein plays an important role in the JAK-STAT signaling pathway which is critical for mediating responses to interferon-gamma. Upon activation by IFN-γ IFNGR1 recruits JAK kinases leading to the phosphorylation of STAT1 a significant transcription factor. STAT1 then dimerizes and translocates to the nucleus where it induces the expression of genes involved in immune defense and inflammation. This process is vital for the body's ability to handle infections and other immunological challenges.
- Associated diseases and disorders
IFNGR1 is associated with susceptibility to infections and certain immune-related disorders. Mutations or deficiencies in IFNGR1 can lead to Mendelian Susceptibility to Mycobacterial Disease (MSMD) where individuals show increased vulnerability to mycobacterial infections. Additionally improper signaling through IFNGR1 is linked to chronic granulomatous disease which can involve defective functioning of the NADPH oxidase complex. Understanding IFNGR1's function and interactions is important for exploring new therapeutic strategies for these disorders.
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2 product images
SDS-PAGE - Recombinant mouse IFNGR1 protein (His tag) (ab276908)
SDS-PAGE analysis of ab276908
Functional Studies - Recombinant mouse IFNGR1 protein (His tag) (ab276908)
Measured by its ability to bind with recombinant mouse IFNG-Fc in a functional ELISA.
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