Recombinant mouse IGF1 protein (Active) is a Full Length protein, in the 33 to 165 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
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Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
Amino acid sequence
G P E T L C G A E L V D A L Q F V C G P R G F Y F N K P T G Y G S S I R R A P Q T G I V D E C C F R S C D L R R L E M Y C A P L K P T K A A R S I R A Q R H T D M P K T Q K S P S L S T N K K T K L Q R R R K G E P K T H P E G E Q E E V T E A T R K I R G P R E K R L G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
IGF IA, IGF IB, IGF-I, IGF1A, IGF1_HUMAN, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor I, MGF, Mechano growth factor, OTTHUMP00000195080, OTTHUMP00000195081, OTTHUMP00000195082, OTTHUMP00000195083, OTTHUMP00000195084, Somatomedia C, Somatomedin-C
Recommended products
Recombinant mouse IGF1 protein (Active) is a Full Length protein, in the 33 to 165 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent increase in MCF7 cell proliferation.
ED50 is ≤47.5 ng/mL, corresponding to a specifc activity of 0.021 x 106 units/mg.
- Animal free
- Yes
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- G P E T L C G A E L V D A L Q F V C G P R G F Y F N K P T G Y G S S I R R A P Q T G I V D E C C F R S C D L R R L E M Y C A P L K P T K A A R S I R A Q R H T D M P K T Q K S P S L S T N K K T K L Q R R R K G E P K T H P E G E Q E E V T E A T R K I R G P R E K R L G
- Protein length
- Full Length
- Predicted molecular weight
- 15.08 kDa
- Actual molecular weight
- 15.08 kDa
- Amino acids
- 33 to 165
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
=95% Purity by HPLC.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The insulin-like growth factor 1 or IGF1 is a protein hormone that plays an important mechanical role in growth and development. Alternative names for IGF1 include somatomedin C. This protein has a mass of approximately 7.6 kDa and is mainly produced by the liver. IGF1 is expressed in a range of tissues including muscle bone and cartilage reflecting its importance in various physiological processes. Its production is stimulated by growth hormone and it acts in an autocrine and paracrine manner to exert its effects.
- Biological function summary
IGF1 stimulates growth and proliferation of cells most notably impacting the development of the skeletal system and the regulation of apoptosis. It is not part of a larger protein complex but it interacts closely with IGF1 receptor to execute its functions. By binding to this receptor IGF1 activates intracellular signaling cascades that promote anabolic effects which include increased uptake of glucose and amino acids contributing to muscle and tissue growth.
- Pathways
IGF1 operates within the PI3K-Akt and MAPK signaling pathways which are significant for cell survival and proliferation. Its interaction with proteins like IGF binding protein 3 (IGFBP3) modulates its availability and activity in the bloodstream. These pathways are critical for mediating the effects of growth hormone highlighting the role of IGF1 in systemic growth and metabolism regulation.
- Associated diseases and disorders
Alterations in IGF1 levels associate with growth abnormalities such as Laron syndrome and acromegaly. Laron syndrome results from the body's inability to use IGF1 properly due to receptor defects while acromegaly occurs from excessive IGF1 production often due to pituitary tumors. Furthermore aberrant IGF1 signaling connects to cancer development where it can drive tumor growth and metastasis. Drugs targeting IGF1 and its pathway components continue to be researched for therapeutic potential in these conditions.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Recombinant mouse IGF1 protein (Active) (ab280348)
Fully biologically active determined by the dose dependent increase in MCF7 cell proliferation.
ED50 is ≤47.5 ng/mL, corresponding to a specifc activity of 0.021 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3379473-1
SDS-PAGE - Recombinant mouse IGF1 protein (Active) (ab280348)
SDS-PAGE analysis of ab280348.
Mass Spectrometry - Recombinant mouse IGF1 protein (Active) (ab280348)
Mass determination by ESI-TOF.
Predicted MW is 15077.3 Da (+/- 10 Da by ESI-TOF). Observed MW is 15077.37. Multiple O-glycosylation sites are present in the sequence and presented in the mass analysis.
HPLC - Recombinant mouse IGF1 protein (Active) (ab280348)
HPLC analysis of ab280348.
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