Recombinant mouse IKK beta protein (Active) is a Mouse Fragment protein, in the 1 to 662 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M S W S P S L P T Q T C G A W E M K E R L G T G G F G N V I R W H N Q A T G E Q I A I K Q C R Q E L S P K N R N R W C L E I Q I M R R L N H P N V V A A R D V P E G M Q N L A P N D L P L L A M E Y C Q G G D L R R Y L N Q F E N C C G L R E G A V L T L L S D I A S A L R Y L H E N R I I H R D L K P E N I V L Q Q G E K R L I H K I I D L G Y A K E L D Q G S L C T S F V G T L Q Y L A P E L L E Q Q K Y T V T V D Y W S F G T L A F E C I T G F R P F L P N W Q P V Q W H S K V R Q K S E V D I V V S E D L N G A V K F S S S L P F P N N L N S V L A E R L E K W L Q L M L M W H P R Q R G T D P Q Y G P N G C F R A L D D I L N L K L V H V L N M V T G T V H T Y P V T E D E S L Q S L K T R I Q E N T G I L E T D Q E L L Q K A G L V L L P D K P A T Q C I S D S K T N E G L T L D M D L V F L L D N S K I N Y E T Q I T P R P P P E S V S C I L Q E P K R N L S F F Q L R K V W G Q V W H S I Q T L K E D C N R L Q Q G Q R A A M M S L L R N N S C L S K M K N A M A S T A Q Q L K A K L D F F K T S I Q I D L E K Y K E Q T E F G I T S D K L L L A W R E M E Q A V E Q C G R E N D V K H L V E R M M A L Q T D I V D L Q R S P M G R K Q G G T L D D L E E Q A R E L Y R K L R E K P R D Q R T E G D S Q E M V R L L L Q A I Q S F E K K V R V I Y T Q L S K T V V C K Q K A L E L L P K V E E V V S L M N E D E R T V V R L Q E K R Q K E L W N L L K I A C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses (By similarity). Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation (By similarity). Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues (By similarity). These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome (By similarity). In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis (By similarity). In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE (By similarity). IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs (By similarity). Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor (By similarity). Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1 (By similarity). Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (PubMed:30988283). Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus (PubMed:25326420). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylates STAT1 at 'Thr-748' which restricts interferon signaling and anti-inflammatory responses and promotes innate inflammatory responses (PubMed:38621137). IKBKB-mediated phosphorylation of STAT1 at 'Thr-748' promotes binding of STAT1 to the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (By similarity). It also promotes binding of STAT1 to the IL12B promoter and activation of IL12B transcription (By similarity).
Alternative names
Ikkb, Ikbkb, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta, Serine/threonine protein kinase IKBKB, IKK2, NFKBIKB
Recommended products
Recombinant mouse IKK beta protein (Active) is a Mouse Fragment protein, in the 1 to 662 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268658 was 48 nmol/min/mg in a kinase assay using IKKtide synthetic peptide as substrate.
- Accession
- O88351-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M S W S P S L P T Q T C G A W E M K E R L G T G G F G N V I R W H N Q A T G E Q I A I K Q C R Q E L S P K N R N R W C L E I Q I M R R L N H P N V V A A R D V P E G M Q N L A P N D L P L L A M E Y C Q G G D L R R Y L N Q F E N C C G L R E G A V L T L L S D I A S A L R Y L H E N R I I H R D L K P E N I V L Q Q G E K R L I H K I I D L G Y A K E L D Q G S L C T S F V G T L Q Y L A P E L L E Q Q K Y T V T V D Y W S F G T L A F E C I T G F R P F L P N W Q P V Q W H S K V R Q K S E V D I V V S E D L N G A V K F S S S L P F P N N L N S V L A E R L E K W L Q L M L M W H P R Q R G T D P Q Y G P N G C F R A L D D I L N L K L V H V L N M V T G T V H T Y P V T E D E S L Q S L K T R I Q E N T G I L E T D Q E L L Q K A G L V L L P D K P A T Q C I S D S K T N E G L T L D M D L V F L L D N S K I N Y E T Q I T P R P P P E S V S C I L Q E P K R N L S F F Q L R K V W G Q V W H S I Q T L K E D C N R L Q Q G Q R A A M M S L L R N N S C L S K M K N A M A S T A Q Q L K A K L D F F K T S I Q I D L E K Y K E Q T E F G I T S D K L L L A W R E M E Q A V E Q C G R E N D V K H L V E R M M A L Q T D I V D L Q R S P M G R K Q G G T L D D L E E Q A R E L Y R K L R E K P R D Q R T E G D S Q E M V R L L L Q A I Q S F E K K V R V I Y T Q L S K T V V C K Q K A L E L L P K V E E V V S L M N E D E R T V V R L Q E K R Q K E L W N L L K I A C
- Accession
- O88351
- Protein length
- Fragment
- Amino acids
- 1 to 662
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses (By similarity). Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation (By similarity). Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues (By similarity). These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome (By similarity). In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis (By similarity). In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE (By similarity). IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs (By similarity). Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor (By similarity). Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1 (By similarity). Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (PubMed:30988283). Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus (PubMed:25326420). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylates STAT1 at 'Thr-748' which restricts interferon signaling and anti-inflammatory responses and promotes innate inflammatory responses (PubMed:38621137). IKBKB-mediated phosphorylation of STAT1 at 'Thr-748' promotes binding of STAT1 to the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (By similarity). It also promotes binding of STAT1 to the IL12B promoter and activation of IL12B transcription (By similarity).
- Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
- Post-translational modifications
Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ; which enhances activity. Phosphorylated by MAP3K7/TAK1 in response to NOD1 and NOD2 signaling, promoting activation and phosphorylation of NF-kappa-B inhibitors, leading to NF-kappa-B activation. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription. Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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2 product images
Functional Studies - Recombinant mouse IKK beta protein (Active) (ab268658)
The specific activity of ab268658 was 48 nmol/min/mg in a kinase assay using IKKtide synthetic peptide as substrate.
SDS-PAGE - Recombinant mouse IKK beta protein (Active) (ab268658)
SDS-PAGE analysis of ab268658.
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