Recombinant mouse IL-1 beta protein (Active) is a Mouse Full Length protein, in the 118 to 269 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture.
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- Nature immunology 24:1149-11602023Conserved stromal-immune cell circuits secure B cell homeostasis and function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMechthild Lütge,Angelina De Martin,Cristina Gil-Cruz,Christian Perez-Shibayama,Yves Stanossek,Lucas Onder,Hung-Wei Cheng,Lisa Kurz,Nadine Cadosch,Charlotte Soneson,Mark D Robinson,Sandro J Stoeckli,Burkhard Ludewig,Natalia B PikorPubMed 37202489
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture
- Biologically active
- Yes
Amino acid sequence
V P I R Q L H Y R L R D E Q Q K S L V L S D P Y E L K A L H L N G Q N I N Q Q V I F S M S F V Q G E P S N D K I P V A L G L K G K N L Y L S C V M K D G T P T L Q L E S V D P K Q Y P K K K M E K R F V F N K I E V K S K V E F E S A E F P N W Y I S T S Q A E H K P V F L G N N S G Q D I I D F T M E S V S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
Alternative names
Interleukin-1 beta, IL-1 beta, Il1b
Recommended products
Recombinant mouse IL-1 beta protein (Active) is a Mouse Full Length protein, in the 118 to 269 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active compared to a standard. ED50 is ≤ 0.5294 ng /ml , corresponding to a specific activity of 1.89 x 106 units/mg.
- Accession
- P10749-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- V P I R Q L H Y R L R D E Q Q K S L V L S D P Y E L K A L H L N G Q N I N Q Q V I F S M S F V Q G E P S N D K I P V A L G L K G K N L Y L S C V M K D G T P T L Q L E S V D P K Q Y P K K K M E K R F V F N K I E V K S K V E F E S A E F P N W Y I S T S Q A E H K P V F L G N N S G Q D I I D F T M E S V S S
- Accession
- P10749
- Protein length
- Full Length
- Predicted molecular weight
- 17.45 kDa
- Amino acids
- 118 to 269
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity by HPLC >=95%.
General info
- Function
Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
- Sequence similarities
Belongs to the IL-1 family.
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
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5 product images
Sandwich ELISA - Recombinant mouse IL-1 beta protein (Active) (ab259421)
Sandwich ELISA - Recombinant mouse IL-1 beta protein standard curve
Background subtracted standard curve using Mouse IL-1beta Antibody Pair - BSA and Azide free (Mouse IL-1beta Antibody Pair - BSA and Azide free ab241673) and Recombinant mouse IL-1 beta protein (Active) (ab259421) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
Functional Studies - Recombinant mouse IL-1 beta protein (Active) (ab259421)
Fully biologically active compared to a standard. Determined by dose-dependant stimulation of human TF-1 cells. ED50 is ≤ 0.5294 ng /ml, corresponding to a specific activity of 1.89 x 106 units/mg.
SDS-PAGE - Recombinant mouse IL-1 beta protein (Active) (ab259421)
SDS-PAGE analysis of ab259421.
HPLC - Recombinant mouse IL-1 beta protein (Active) (ab259421)
Purity 100%
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
Mass Spectrometry - Recombinant mouse IL-1 beta protein (Active) (ab259421)
M + 0.61 Da (Calc. mass 17451.39).
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
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