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Recombinant mouse IL-1 beta protein (Active) is a Mouse Full Length protein, in the 118 to 269 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture.

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Images

Sandwich ELISA - Recombinant mouse IL-1 beta protein (Active) (AB259421), expandable thumbnail
  • Functional Studies - Recombinant mouse IL-1 beta protein (Active) (AB259421), expandable thumbnail
  • SDS-PAGE - Recombinant mouse IL-1 beta protein (Active) (AB259421), expandable thumbnail
  • HPLC - Recombinant mouse IL-1 beta protein (Active) (AB259421), expandable thumbnail
  • Mass Spectrometry - Recombinant mouse IL-1 beta protein (Active) (AB259421), expandable thumbnail

Publications

Key facts

Purity
>=95% SDS-PAGE
Endotoxin level
<= 0.005 EU/µg
Expression system
HEK 293 cells
Tags
Tag free
Applications
SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture
Biologically active
Yes

Amino acid sequence

V P I R Q L H Y R L R D E Q Q K S L V L S D P Y E L K A L H L N G Q N I N Q Q V I F S M S F V Q G E P S N D K I P V A L G L K G K N L Y L S C V M K D G T P T L Q L E S V D P K Q Y P K K K M E K R F V F N K I E V K S K V E F E S A E F P N W Y I S T S Q A E H K P V F L G N N S G Q D I I D F T M E S V S S

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Cell Culture
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

Alternative names

Recommended products

Recombinant mouse IL-1 beta protein (Active) is a Mouse Full Length protein, in the 118 to 269 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture.

Key facts

Purity
>=95% SDS-PAGE
Endotoxin level
<= 0.005 EU/µg
Expression system
HEK 293 cells
Applications
SDS-PAGE, FuncS, MS, HPLC, sELISA, Cell Culture
Biological activity
Fully biologically active compared to a standard. ED50 is ≤ 0.5294 ng /ml , corresponding to a specific activity of 1.89 x 106 units/mg.
Accession
P10749-1
Animal free
Yes
Species
Mouse
Reconstitution
Reconstitute in PBS
Concentration
Loading...
Storage buffer

pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

V P I R Q L H Y R L R D E Q Q K S L V L S D P Y E L K A L H L N G Q N I N Q Q V I F S M S F V Q G E P S N D K I P V A L G L K G K N L Y L S C V M K D G T P T L Q L E S V D P K Q Y P K K K M E K R F V F N K I E V K S K V E F E S A E F P N W Y I S T S Q A E H K P V F L G N N S G Q D I I D F T M E S V S S
Accession
P10749
Protein length
Full Length
Predicted molecular weight
17.45 kDa
Amino acids
118 to 269
Nature
Recombinant

Specifications

Form
Lyophilized
Additional notes

Purity by HPLC >=95%.

General info

Function

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

Sequence similarities

Belongs to the IL-1 family.

Subcellular localisation
Lysosome

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Sandwich ELISA - Recombinant mouse IL-1 beta protein (Active) (ab259421), expandable thumbnail

    Sandwich ELISA - Recombinant mouse IL-1 beta protein (Active) (ab259421)

    Sandwich ELISA - Recombinant mouse IL-1 beta protein standard curve

    Background subtracted standard curve using Mouse IL-1beta Antibody Pair - BSA and Azide free (Mouse IL-1beta Antibody Pair - BSA and Azide free ab241673) and Recombinant mouse IL-1 beta protein (Active) (ab259421) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.

  • Functional Studies - Recombinant mouse IL-1 beta protein (Active) (ab259421), expandable thumbnail

    Functional Studies - Recombinant mouse IL-1 beta protein (Active) (ab259421)

    Fully biologically active compared to a standard. Determined by dose-dependant stimulation of human TF-1 cells. ED50 is ≤ 0.5294 ng /ml, corresponding to a specific activity of 1.89 x 106 units/mg.

  • SDS-PAGE - Recombinant mouse IL-1 beta protein (Active) (ab259421), expandable thumbnail

    SDS-PAGE - Recombinant mouse IL-1 beta protein (Active) (ab259421)

    SDS-PAGE analysis of ab259421.

  • HPLC - Recombinant mouse IL-1 beta protein (Active) (ab259421), expandable thumbnail

    HPLC - Recombinant mouse IL-1 beta protein (Active) (ab259421)

    Purity 100%

    The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.

  • Mass Spectrometry - Recombinant mouse IL-1 beta protein (Active) (ab259421), expandable thumbnail

    Mass Spectrometry - Recombinant mouse IL-1 beta protein (Active) (ab259421)

    M + 0.61 Da (Calc. mass 17451.39).

    The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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