Recombinant mouse IL-10 protein (Active)
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Recombinant mouse IL-10 protein (Active) is a Mouse Full Length protein, in the 19 to 178 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.
View Alternative Names
Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF
- FuncS
Supplier Data
Functional Studies - Recombinant mouse IL-10 protein (Active) (AB281800)
Functional analysis of ab281800.
Fully biologically active determined by dose dependent increase in proliferation of MC/9 cells.
ED50 for this effect is ≤ 0.330 ng/ml corresponding to a specific activity of 3.03 X 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot : GR3387982-1
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant mouse IL-10 protein (Active) (AB281800)
Mass determination by ESI-TOF.
Predicted MW is 18810.75 Da. (+/- 10 Da by ESI-TOF). Observed MW is 18812.07 Da.
- HPLC
Supplier Data
HPLC - Recombinant mouse IL-10 protein (Active) (AB281800)
HPLC analysis of ab281800.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant mouse IL-10 protein (Active) (AB281800)
SDS-PAGE analysis of ab281800.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Specifications
Form
Lyophilized
Additional notes
Equal or greater than 95% by HPLC
General info
Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed : 28473584).
Sequence similarities
Belongs to the IL-10 family.
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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