Recombinant mouse IL-11 protein (Active) is a Mouse Full Length protein, in the 22 to 199 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
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Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
Amino acid sequence
P G P P A G S P R V S S D P R A D L D S A V L L T R S L L A D T R Q L A A Q M R D K F P A D G D H S L D S L P T L A M S A G T L G S L Q L P G V L T R L R V D L M S Y L R H V Q W L R R A G G P S L K T L E P E L G A L Q A R L E R L L R R L Q L L M S R L A L P Q A A P D Q P V I P L G P P A S A W G S I R A A H A I L G G L H L T L D W A V R G L L L L K T R L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed:8913282). Also promotes the proliferation of hepatocytes in response to liver damage (PubMed:22253262). Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation, also in the context of various cancers (PubMed:10026196, PubMed:23948300). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3 (PubMed:22253262, PubMed:23948300). The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (By similarity).
Alternative names
Interleukin-11, IL-11, Il11
Recommended products
Recombinant mouse IL-11 protein (Active) is a Mouse Full Length protein, in the 22 to 199 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent proliferation of TF-1 cells.
ED50 for this effect is ≤12.55 ng/ml, corresponding to a specific activity of 0.80 x 105 units/mg.
- Accession
- P47873-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- P G P P A G S P R V S S D P R A D L D S A V L L T R S L L A D T R Q L A A Q M R D K F P A D G D H S L D S L P T L A M S A G T L G S L Q L P G V L T R L R V D L M S Y L R H V Q W L R R A G G P S L K T L E P E L G A L Q A R L E R L L R R L Q L L M S R L A L P Q A A P D Q P V I P L G P P A S A W G S I R A A H A I L G G L H L T L D W A V R G L L L L K T R L
- Accession
- P47873
- Protein length
- Full Length
- Predicted molecular weight
- 19.21 kDa
- Actual molecular weight
- 19.21 kDa
- Amino acids
- 22 to 199
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>=95% purity by HPLC.
General info
- Function
Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed:8913282). Also promotes the proliferation of hepatocytes in response to liver damage (PubMed:22253262). Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation, also in the context of various cancers (PubMed:10026196, PubMed:23948300). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3 (PubMed:22253262, PubMed:23948300). The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (By similarity).
- Sequence similarities
Belongs to the IL-6 superfamily.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Recombinant mouse IL-11 protein (Active) (ab281802)
Functional analysis of ab281802.
Fully biologically active determined by the dose dependent proliferation of TF-1 cells.
ED50 for this effect is ≤12.55 ng/ml, corresponding to a specific activity of 0.80 x 105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3390483-1
SDS-PAGE - Recombinant mouse IL-11 protein (Active) (ab281802)
SDS-page analysis of ab281802.
Mass Spectrometry - Recombinant mouse IL-11 protein (Active) (ab281802)
Mass determination by ESI-TOF.
Predicted MW is 19211.47 Da (+/- 10 Da by ESI-TOF). Observed MW is 19212.35.
HPLC - Recombinant mouse IL-11 protein (Active) (ab281802)
HPLC analysis of ab281802.
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