Recombinant mouse IL-12 protein (Active) is a Mouse Full Length protein, in the 23 to 215 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.
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Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
< 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, Cell Culture, HPLC
- Biologically active
Yes
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
R V I P V S G P A R C L S Q S R N L L K T T D D M V K T A R E K L K H Y S C T A E D I D H E D I T R D Q T S T L K T C L P L E L H K N E S C L A T R E T S S T T R G S C L P P Q K T S L M M T L C L G S I Y E D L K M Y Q T E F Q A I N A A L Q N H N H Q Q I I L D K G M L V A I D E L M Q S L N H N G E T L R Q K P P V G E A D P Y R V K M K L C I L L H A F S T R V V T I N R V M G Y L S S A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Targets
Il12b
Alternative names
Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, CLMF p35, Il12a
Recommended products
Recombinant mouse IL-12 protein (Active) is a Mouse Full Length protein, in the 23 to 215 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, Cell Culture, HPLC.
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
< 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, Cell Culture, HPLC
- Biologically active
Yes
- Biological activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant Proliferation of CTLL-2 cells is 1.96 ng/mL corresponding to a Specific Activity of 5.10 x 105 IU/mg.
- Accession
- P43431-1
- Animal free
Yes
- Species
Mouse
- Reconstitution
Reconstitute with Phosphate Buffered saline.Reconstituted protein stable at -80C for 12 months or 4C for 1 week. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence 1
R V I P V S G P A R C L S Q S R N L L K T T D D M V K T A R E K L K H Y S C T A E D I D H E D I T R D Q T S T L K T C L P L E L H K N E S C L A T R E T S S T T R G S C L P P Q K T S L M M T L C L G S I Y E D L K M Y Q T E F Q A I N A A L Q N H N H Q Q I I L D K G M L V A I D E L M Q S L N H N G E T L R Q K P P V G E A D P Y R V K M K L C I L L H A F S T R V V T I N R V M G Y L S S A
- Accession
- Protein length
Full Length
- Predicted molecular weight
21.76 kDa
- Amino acids
23 to 215
- Nature
Recombinant
Amino acid sequence 2
M W E L E K D V Y V V E V D W T P D A P G E T V N L T C D T P E E D D I T W T S D Q R H G V I G S G K T L T I T V K E F L D A G Q Y T C H K G G E T L S H S H L L L H K K E N G I W S T E I L K N F K N K T F L K C E A P N Y S G R F T C S W L V Q R N M D L K F N I K S S S S S P D S R A V T C G M A S L S A E K V T L D Q R D Y E K Y S V S C Q E D V T C P T A E E T L P I E L A L E A R Q Q N K Y E N Y S T S F F I R D I I K P D P P K N L Q M K P L K N S Q V E V S W E Y P D S W S T P H S Y F S L K F F V R I Q R K K E K M K E T E E G C N Q K G A F L V E K T S T E V Q C K G G N V C V Q A Q D R Y Y N S S C S K W A C V P C R V R S
- Accession
- Protein length
Full Length
- Predicted molecular weight
35.85 kDa
- Amino acids
23 to 335
- Nature
Recombinant
Specifications
- Form
Lyophilized
- Additional notes
>= 95 % HPLC.
General info
- Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
- Sequence similarities
Belongs to the IL-6 superfamily.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
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4 product images
Functional Studies - Recombinant mouse IL-12 protein (Active) (ab259419)
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant Proliferation of CTLL-2 cells is 1.96 ng/mL corresponding to a Specific Activity of 5.10 x 105 IU/mg.
SDS-PAGE - Recombinant mouse IL-12 protein (Active) (ab259419)
SDS-PAGE analysis of ab259419.
HPLC - Recombinant mouse IL-12 protein (Active) (ab259419)
Purity: 100% (heterodimer)
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
Mass Spectrometry - Recombinant mouse IL-12 protein (Active) (ab259419)
M + 0. 9 Da (calc mass 21762.1) and M + 1.6 Da (calc mass 35848.4); heterodimer
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
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