JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB270080

Recombinant mouse IL-13 protein (Active)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant mouse IL-13 protein (Active) is a Mouse Full Length protein, in the 19 to 131 aa range, expressed in HEK 293 cells, with >95%, < 0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

View Alternative Names

Il-13, Il13, Interleukin-13, IL-13, T-cell activation protein P600

4 Images
Mass Spectrometry - Recombinant mouse IL-13 protein (Active) (AB270080)
  • Mass Spec

Lab

Mass Spectrometry - Recombinant mouse IL-13 protein (Active) (AB270080)

M+1.63 Da, pass (+655 Da : Hex(1)HexNAc(1)NeuAc(1)). Calc MW 12409.37 Da

Functional Studies - Recombinant mouse IL-13 protein (Active) (AB270080)
  • FuncS

Lab

Functional Studies - Recombinant mouse IL-13 protein (Active) (AB270080)

Fully biologically active when compared to standard. Determined by the dose dependant proliferation of TF-1 cell line.

ED50 is ≤ 1.892 ng/mL, corresponding to a specific activity of 5.29 x 105 units/mg.

HPLC - Recombinant mouse IL-13 protein (Active) (AB270080)
  • HPLC

Lab

HPLC - Recombinant mouse IL-13 protein (Active) (AB270080)

Purity by HPLC ≥95%.

SDS-PAGE - Recombinant mouse IL-13 protein (Active) (AB270080)
  • SDS-PAGE

Lab

SDS-PAGE - Recombinant mouse IL-13 protein (Active) (AB270080)

SDS-PAGE analysis of ab270080.

The protein has multiple N-linked glycosylations.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, HPLC, FuncS, Cell Culture, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Fully biologically active when compared to standard. Determined by the dose dependant proliferation of TF-1 cell line.

ED50 is ≤ 1.892 ng/mL, corresponding to a specific activity of 5.29 x 105 units/mg.

Accession

P20109

Animal free

No

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 6 - 8 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
style
left-column

Sequence info

[{"sequence":"APGPVPRSVSLPLTLKELIEELSNITQDQTPLCNGSMVWSVDLAAGGFCVALDSLTNISNCNAIYRTQRILHGLCNRKAPTTVSSLPDTKIEVAHFITKLLSYTKQLFRHGPF","proteinLength":"Full Length","predictedMolecularWeight":"12.41 kDa","actualMolecularWeight":null,"aminoAcidEnd":131,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P20109","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
style
left-column
style
left-column

Specifications

Form

Lyophilized

Additional notes

Purity by HPLC >=95%.

style
left-column

General info

Function

Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed : 15361238). Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (By similarity). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (PubMed : 34789557). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Functions also on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils. Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed : 34795444, PubMed : 8871614). Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed : 29305434).

Sequence similarities

Belongs to the IL-4/IL-13 family.

style
left-column

Product protocols

style
left-column

Target data

Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed : 15361238). Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (By similarity). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (PubMed : 34789557). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Functions also on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils. Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed : 34795444, PubMed : 8871614). Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed : 29305434).
See full target information Il13
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB155733

Recombinant human IL-4 protein

Bioactive

Functional Studies - Recombinant human IL-4 protein (AB155733)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB259402

Recombinant human IL-10 protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human IL-10 protein (Active) (AB259402)

  • 0
  • 0
Proteins & Peptides

AB259381

Recombinant human IL-6 protein (Active)

Premium Bioactive

Functional Studies (Neut/Block) - Recombinant human IL-6 protein (Active) (AB259381)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com