Recombinant mouse IL-13 protein (Active) is a Mouse Full Length protein, in the 19 to 131 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
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Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
A P G P V P R S V S L P L T L K E L I E E L S N I T Q D Q T P L C N G S M V W S V D L A A G G F C V A L D S L T N I S N C N A I Y R T Q R I L H G L C N R K A P T T V S S L P D T K I E V A H F I T K L L S Y T K Q L F R H G P F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed:15361238). Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (By similarity). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (PubMed:34789557). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Functions also on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils. Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed:34795444, PubMed:8871614). Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed:29305434).
Alternative names
Il-13, Il13, Interleukin-13, IL-13, T-cell activation protein P600
Recommended products
Recombinant mouse IL-13 protein (Active) is a Mouse Full Length protein, in the 19 to 131 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active when compared to standard. Determined by the dose dependant proliferation of TF-1 cell line.
ED50 is ≤ 1.892 ng/mL, corresponding to a specific activity of 5.29 x 105 units/mg.
- Accession
- P20109-1
- Animal free
- No
- Species
- Mouse
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A P G P V P R S V S L P L T L K E L I E E L S N I T Q D Q T P L C N G S M V W S V D L A A G G F C V A L D S L T N I S N C N A I Y R T Q R I L H G L C N R K A P T T V S S L P D T K I E V A H F I T K L L S Y T K Q L F R H G P F
- Accession
- P20109
- Protein length
- Full Length
- Predicted molecular weight
- 12.41 kDa
- Amino acids
- 19 to 131
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity by HPLC >=95%.
General info
- Function
Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed:15361238). Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (By similarity). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (PubMed:34789557). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Functions also on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils. Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed:34795444, PubMed:8871614). Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed:29305434).
- Sequence similarities
Belongs to the IL-4/IL-13 family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
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4 product images
Functional Studies - Recombinant mouse IL-13 protein (Active) (ab270080)
Fully biologically active when compared to standard. Determined by the dose dependant proliferation of TF-1 cell line.
ED50 is ≤ 1.892 ng/mL, corresponding to a specific activity of 5.29 x 105 units/mg.
SDS-PAGE - Recombinant mouse IL-13 protein (Active) (ab270080)
SDS-PAGE analysis of ab270080.The protein has multiple N-linked glycosylations.
HPLC - Recombinant mouse IL-13 protein (Active) (ab270080)
Purity by HPLC ≥95%.
Mass Spectrometry - Recombinant mouse IL-13 protein (Active) (ab270080)
M+1.63 Da, pass (+655 Da: Hex(1)HexNAc(1)NeuAc(1)). Calc MW 12409.37 Da
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